Volume 18, Number 10—October 2012
Dispatch
Human Polyomaviruses in Children Undergoing Transplantation, United States, 2008–2010
Erica A. Siebrasse, Irma Bauer, Lori R. Holtz, Binh-minh Le, Sherry Lassa-Claxton, Charles Canter, Paul Hmiel, Shalini Shenoy, Stuart Sweet, Yumirle Turmelle, Ross Shepherd, and David Wang
Table 1
Real-time PCR assays to detect human polyomaviruses in children undergoing transplants, United States, 2008–2010*
| Virus | Target | Primers, 5′ → 3′ | Probe, 5′ → 3′ |
|---|---|---|---|
| WUPyV | NCCR | WU-C-4824-F: GGCACGGCGCCAACT | WU-C-4861-TM: FAM-TGCCATACCAACACAGCTGCTGAGC-TAMRA-3′ |
| WU-C-4898-R: CCTGTTGTAGGCCTTACTTACCTGTA | |||
| KIPyV | LTAg | KI-B-4603-F: GAATGCATTGGCATTCGTGA | KI-B-4632-TM: FAM-TGTAGCCATGAATGCATACATCCCACTGC-TAMRA |
| KI-B-4668-R: GCTGCAATAAGTTTAGATTAGTTGGTGC | |||
| TSPyV | LTAg | LTF: TGTGTTTGGAAACCAGAATCATTTG | LTP: FAM-TTCTTCTTCCTCCTCATCCTCCACCTCAAT-TAMRA |
| LTR: TGCTACCTTGCTATTAAATGTGGAG | |||
| MCPyV | LTAg | LT.1F: CCACAGCCAGAGCTCTTCCT | LT probe: FAM-TCCTTCTCAGCGTCCCAGGCTTCA-TAMRA |
| LT.1R: TGGTGGTCTCCTCTCTGCTACTG | |||
| HPyV6 | VP1 | ES011F: GCCTGGAAGGGCCTAGTAAAG | ES024: FAM-ACCAACCATCTGTTGCAGGCATTAAAGCTA-TAMRA |
| ES012R: ATTGGCAGCTGTAACTTGTTTTCTG | |||
| HPyV7 | VP1 | ES017F: GGTCCAGGCAATACTGATGTAGCTA | ES025: FAM-CCTGCAAGCCCTCAGAAAGCAAGTAAATG-TAMRA |
| ES018R: TCTGCAACCCAGAGCTCTACTG | |||
| HPyV9 | LTAg | ES026F: GAAGACCCTGATCCTGAGGAAGA | ES031: FAM-TGGATCATCCCAGAGTTCATTTACCTGCA-TAMRA |
| ES027R: CTCTGGAGTATTAGGTTCAGGCTTCT | |||
| BKPyV | LTAg | BK-Deg-F: AGCAGGCAAGRGTTCTATTACTAAAT | Bk-Deg-P: FAM-AAGACCCTAAAGACTTTCCYTCTGATCTACACCAGTTT-TAMRA |
| BK-Deg-R: GARGCAACAGCAGATTCYCAACA | |||
| JCPyV | VP2/3 | JL1 (F): AAGGGAGGGAACCTATATTTCTTTTG | JL1 (P): FAM-CTCATACACCCAAAGTATAGATGATGCAGACAGCA-TAMRA |
| JL1 (R): TCTAGCCTTTGGGTAACTTCTTGAA |
*WUPyV, WU polyomavirus; NCCR, non-coding control region; KIPyV, KI polyomavirus; LTAg, large T antigen; TSPyV, trichodysplasia spinulosa polyomavirus; MCPyV, Merkel cell polyomavirus; HPyV, human polyomavirus; VP, virion protein; BKPyV, BK polyomavirus; JCPyV, JC polyomavirus.
