Volume 18, Number 12—December 2012
Research
Borrelia, Rickettsia, and Ehrlichia Species in Bat Ticks, France, 2010
Cristina Socolovschi, Tahar Kernif, Didier Raoult, and Philippe Parola
Table
Primers and probes used to detect and confirm the presence of bacteria in Argas vespertilionis ticks collected in 2010 from an attic in France*
| Bacterial species | Gene target (ref) | Primers and probe |
|---|---|---|
| Screening | ||
| Rickettsia | RC0338 (12) | 1029-F1: 5′-GAM AAA TGA ATT ATA TAC GCC GCA AA-3′ 1029-R1: 5′-ATT ATT KCC AAA TAT TCG TCC TGT AC-3′ 1029–1P: 6FAM-CTC AAG ATA AGT ATG AGT TAA ATG TAA A-TAMRA |
| Borrelia | 16S rRNA (13) | Bor_16S_3_F: 5′ AGC CTT TAA AGC TTC GCT TGT AG 3′ Bor_16S_3_R: 5′ GCC TCC CGT AGG AGT CTG G 3′ Bor_16S_3_P: 6FAM- CCG GCC TGA GAG GGT GAA CGG TAMRA |
| Coxiella burnetii | IS1111 (14) | IS 1111F: 5′-CAA GAA ACG TAT CGC TGT GGC-3′ IS 1111R: 5′-CAC AGA GCC ACC GTA TGA ATC-3′ IS1111P: 6-FAM-CCG AGT TCG AAA CAA TGA GGG CTG-TAMRA |
| Bartonella | ITS (15) | Barto ITS3 F: 5′-GAT GCC GGG GAA GGT TTT C-3′ Barto ITS3 R: 5′-GCC TGG GAG GAC TTG AAC CT-3′ Barto ITS3 P: 6 FAM-GCG CGC GCT TGA TAA GCG TG-TAMRA |
| Ehrlichia | 16S rRNA (16) | Erli_16S_F: 5′-GGT-ACC-YAC-AGA-AGA-AGT-CC-3′ Erli_16S_R: 5′-TAG-CAC-TCA-TCG-TTT-ACA-GC-3′ |
| Confirmation |
||
| Rickettsia | gltA (17) | 409D: 5′-CCT ATG GCT ATT ATG CTT GC-3′ 1258R: 5′-ATT GCA AAA AGT ACA GTG AAC A-3′ |
| Rickettsia | ompA (17) | 190–70: 5′-ATG GCG AAT ATT TCT CCA AAA-3′ 190–701: 5′- GTT CCG TTAATGGCAGCA TCT-3′ 190–180: 5′- GCA GCG ATA ATG CTG AGT A-3′ |
| Borrelia | 16S rRNA (13) | BF1: 5′-GCT GGC AGT GCG TCT TAA GC-3′ BR1: 5′-GCT TCG GGT ATC CTC AAC TC-3′ |
| Borrelia | Flagellin (18) | Bor1: 5′- TAA TAC GTC AGC CAT AAA TGC-3′ Bor2: 5′- GCT CTT TGA TCA GTT ATC ATT C-3′ |
| C. burnetii | IS30 (14) | IS30a F: 5′-CGC TGA CCT ACA GAA ATA TGT CC-3′ IS30a R: 5′-GGG GTA AGT AAA TAA TAC CTT CTG G-3′ IS30a P: 6-FAM-CAT GAA GCG ATT TAT CAA TAC GTG TAT GC-TAMRA |
| Bartonella | ITS (15) | Urbarto1: 5′-CTT-CGT-TTC-TCT-TTC-TTC-A-3′ Urbarto2: 5′-CTT-CTC-TTC-ACA-ATT-TCA-AT-3′ |
| Ehrlichia | gltA (19) | HER-CS133F: 5′-GGW TTY ATG TCY ACT GCT GC-3′ HER-CS778R: 5′-GCN CCM CCA TGM GCT GG-3′ |
*Ref, reference. Primers in boldface were used for sequencing.
