Volume 18, Number 12—December 2012
Dispatch
Variant Rabbit Hemorrhagic Disease Virus in Young Rabbits, Spain
Kevin P. Dalton, Inés Nicieza, Ana Balseiro, María A. Muguerza, Joan M. Rosell, Rosa Casais, Ángel L. Álvarez, and Francisco Parra
Table 2
Immunohistochemical detection of RHDV-N11 in tissues from experimentally infected and control rabbits by using monoclonal antibody 6G2*
| Rabbit no. and age | Infection method | Tissue |
|||||
|---|---|---|---|---|---|---|---|
| Liver | Lung | Kidney | Spleen | Intestine | Heart | ||
| 1. Adult (control) | SC | – | – | – | – | +/– | – |
| 2. Young (control)† | SC | + | ++ | ++ | + | ++ | +/– |
| 3. Young | SC | – | – | – | – | – | – |
| 4. Adult‡ | SC | + | + | ++ | +++ | + | +/– |
| 5. Young‡ | SC | +++ | + | ++ | +++ | ++ | +/– |
| 6. Adult | SC | – | – | – | – | – | – |
| 7. Adult | IN | – | – | – | – | + | – |
| 8. Young | IN | – | – | – | – | – | – |
*Young rabbits were 30 d of age on the day of experimental infection. Control rabbits (1 and 2) were each injected with 500 µL sterile PBS. RHDV, rabbit hemorrhagic disease virus; PBS, phosphate-buffered saline; SC, subcutaneous injection; IN, intranasal administration; –, not detected; +, ++, +++, increasing levels of antigen.
†Died 96 h after experimental infection of animals 3–8.
‡Died 48 h after experimental infection.
