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Volume 18, Number 12—December 2012

Dispatch

Differentiation of Prions from L-type BSE versus Sporadic Creutzfeldt-Jakob Disease

Simon Nicot, Anna Bencsik, Eric Morignat, Nadine Mestre-Francés, Armand Perret-Liaudet, and Thierry BaronComments to Author 
Author affiliations: Author affiliations: Agence Nationale de Sécurité Sanitaire (Anses), Lyon, France (S. Nicot, A. Bencsik, E. Morignat, T. Baron); INSERM U710, Montpellier, France; Université Montpellier 2, Montpellier; École Pratique des Hautes Études, Paris, France (N. Mestre-Francés); Hôpitaux Civils de Lyon, Université Lyon 1, INSERM U1028, and Centre National de la Recherche Scientifique, Lyon (A. Perret-Liaudet)

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Figure 1

Susceptibility of Syrian golden hamsters to MM2-cortical subtype sporadic Creutzfeldt-Jakob disease (sCJD) and L-type bovine spongiform encephalopathy (L-BSE) prions. Disease-associated prion protein (PrPd) was analyzed in brains of hamsters injected with human MM2-cortical sCJD and L-BSE from a mouse lemur by paraffin-embedded tissue blot (A, B), immunohistochemistry (C, D), or Western blot (E, F). Monoclonal antibodies against prion protein were SAF84 (A–D), SHa31 (E), and 12B2 (F). C, D) Scal

Figure 1. . . . Susceptibility of Syrian golden hamsters to MM2-cortical subtype sporadic Creutzfeldt-Jakob disease (sCJD) and L-type bovine spongiform encephalopathy (L-BSE) prions. Disease-associated prion protein (PrPd) was analyzed in brains of hamsters injected with human MM2-cortical sCJD and L-BSE from a mouse lemur by paraffin-embedded tissue blot (A, B), immunohistochemistry (C, D), or Western blot (E, F). Monoclonal antibodies against prion protein were SAF84 (A–D), SHa31 (E), and 12B2 (F). C, D) Scale bars = 200 µm. E, F) Controls were hamsters infected with L-BSE from cattle (isolate 02-2528) and with scrapie (experimental isolate SSBP/1 after a first passage in ovine prion protein–transgenic mice). Lane 1, sCJD MM2; lane 2, L-BSE from lemur; lane 3, L-BSE from cattle control; lane 4, scrapie control. Bars to the right indicate the 29.0- and 20.1-kDa marker positions.

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