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Volume 18, Number 12—December 2012

Research

Virulent Avian Infectious Bronchitis Virus, People’s Republic of China

Jinling Feng, Yanxin Hu, Zhijun Ma, Qi Yu, Jixun Zhao, Xiaodong Liu, and Guozhong ZhangComments to Author 
Author affiliations: Author affiliations: The Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, People’s Republic of China (J.L. Feng, Y.X. Hu, J.X. Zhao, G.Z. Zhang); Animal Husbandry and Veterinary Station of Beijing, Beijing (Z.J. Ma, Q. Yu, X.D. Liu)

Main Article

Table 3

Tissue tropism of YN strain of avian IBV, People’s Republic of China*

Method Tissue examined†
Trachea Lung Kidney Bursa Brain Liver
RT-PCR‡ 10/10 10/10 8/10 7/10 1/10 1/10
Virus isolation§ 7/10 8/10 10/10 9/10 ND ND

*Thirty-day-old SPF chickens were inoculated intranasally with YN IBV (105 50% egg infectious dose per bird). IBV, infectious bronchitis virus; SPF, specific-pathogen free; RT-PCR, reverse transcription PCR; ND, not detected.
†Ten tissue samples from euthanized birds were randomly collected and examined.
‡RT-PCR was directly performed on collected tissues.
§Tissue samples were collected for re-isolation of the challenge virus using SPF embryos. Data are the number of tissues from which the virus was isolated/number of tissues examined.

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