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Volume 18, Number 12—December 2012

Research

Diagnostic Assays for Crimean-Congo Hemorrhagic Fever

Jessica VanhomwegenComments to Author , Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, Helen Karlberg, Gülay Korukluoğlu, Miša Korva, Masoud Mardani, Ali Mirazimi, Mehrdad Mousavi, Anna Papa, Ana Saksida, Batool Sharifi-Mood, Persofoni Sidira, Katerina Tsergouli, Roman Wölfel, Hervé Zeller, and Philippe Dubois
Author affiliations: Author affiliations: Institut Pasteur, Paris, France (J. Vanhomwegen, P. Dubois); European Centre for Disease Control, Stockholm, Sweden (J. Vanhomwegen, H. Zeller); National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal (M.J. Alves); Faculty of Medicine, Ljubljana, Slovenia (T. Avšič Županc, M. Korva, A. Saksida); Institute of Public Health, Tirana, Albania (S. Bino); Pasteur Institute of Iran, Tehran, Iran (S. Chinikar); Swedish Institute for Infectious Disease Control, Solna, Sweden (H. Karlberg, A. Mirazimi, M. Mousavi); Refik Saydam National Public Health Agency, Ankara, Turkey (G. Korukluoğlu); Shahid Beheshti University of Medical Sciences, Tehran, Iran (M. Mardani, B. Sharifi-Mood); Aristotle University of Thessaloniki, Thessaloniki, Greece (A. Papa, P. Sidira, K. Tsergouli); and Bundeswehr Institute of Microbiology, Munich, Germany (R. Wölfel)

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Table 1

Reference methods used by the reference laboratories that participated in evaluation of serologic and molecular assays for diagnosis of CCHF

Laboratory Reference IgM serologic tests Reference IgG serologic tests Reference molecular tests
1 In-house IFA (CCHFV-infected cells) In-house IFA (CCHFV-infected cells) Nested RT-PCR (23) and qRT-PCR (24)
2 In-house IgM capture ELISA (CCHFV strain IbAr10200 antigen) In-house sandwich ELISA (CCHFV strain IbAr10200 antigen) Nested RT-PCR (25) and qRT-PCR (17)
3 In-house IFA (CCHFV strain ArD39554 infected cells) In-house IFA (CCHF strain ArD39554 infected cells) qRT-PCR (18)
4 In-house IFA (CCHFV infected cells) In-house IFA (CCHF infected cells) qRT-PCR (18)
5 Testing performed by external reference laboratory Testing performed by external reference laboratory qRT-PCR (18)

*CCHF, Crimean-Congo hemorrhagic fever; IFA, immunofluorescence assay; CCHFV, CCHF virus; RT-PCR, reverse transcription PCR; qRT-PCR, quantitative RT-PCR.

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