Volume 18, Number 12—December 2012
Research
Diagnostic Assays for Crimean-Congo Hemorrhagic Fever
Jessica Vanhomwegen
, Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, Helen Karlberg, Gülay Korukluoğlu, Miša Korva, Masoud Mardani, Ali Mirazimi, Mehrdad Mousavi, Anna Papa, Ana Saksida, Batool Sharifi-Mood, Persofoni Sidira, Katerina Tsergouli, Roman Wölfel, Hervé Zeller, and Philippe Dubois
, Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, Helen Karlberg, Gülay Korukluoğlu, Miša Korva, Masoud Mardani, Ali Mirazimi, Mehrdad Mousavi, Anna Papa, Ana Saksida, Batool Sharifi-Mood, Persofoni Sidira, Katerina Tsergouli, Roman Wölfel, Hervé Zeller, and Philippe DuboisTable 1
Reference methods used by the reference laboratories that participated in evaluation of serologic and molecular assays for diagnosis of CCHF
| Laboratory | Reference IgM serologic tests | Reference IgG serologic tests | Reference molecular tests |
|---|---|---|---|
| 1 | In-house IFA (CCHFV-infected cells) | In-house IFA (CCHFV-infected cells) | Nested RT-PCR (23) and qRT-PCR (24) |
| 2 | In-house IgM capture ELISA (CCHFV strain IbAr10200 antigen) | In-house sandwich ELISA (CCHFV strain IbAr10200 antigen) | Nested RT-PCR (25) and qRT-PCR (17) |
| 3 | In-house IFA (CCHFV strain ArD39554 infected cells) | In-house IFA (CCHF strain ArD39554 infected cells) | qRT-PCR (18) |
| 4 | In-house IFA (CCHFV infected cells) | In-house IFA (CCHF infected cells) | qRT-PCR (18) |
| 5 | Testing performed by external reference laboratory | Testing performed by external reference laboratory | qRT-PCR (18) |
*CCHF, Crimean-Congo hemorrhagic fever; IFA, immunofluorescence assay; CCHFV, CCHF virus; RT-PCR, reverse transcription PCR; qRT-PCR, quantitative RT-PCR.
