Volume 18, Number 12—December 2012
Research
Diagnostic Assays for Crimean-Congo Hemorrhagic Fever
Jessica Vanhomwegen
, Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, Helen Karlberg, Gülay Korukluoğlu, Miša Korva, Masoud Mardani, Ali Mirazimi, Mehrdad Mousavi, Anna Papa, Ana Saksida, Batool Sharifi-Mood, Persofoni Sidira, Katerina Tsergouli, Roman Wölfel, Hervé Zeller, and Philippe Dubois
, Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, Helen Karlberg, Gülay Korukluoğlu, Miša Korva, Masoud Mardani, Ali Mirazimi, Mehrdad Mousavi, Anna Papa, Ana Saksida, Batool Sharifi-Mood, Persofoni Sidira, Katerina Tsergouli, Roman Wölfel, Hervé Zeller, and Philippe DuboisTable 4
Overall performance of assays compared in study of CCHF diagnostic tools
| Parameter | IgM serology |
IgG serology |
Genome detection |
|||||
|---|---|---|---|---|---|---|---|---|
| ELISA | IFA | ELISA | IFA | qRT-PCR | LCD array | |||
| No. samples tested | 138 | 90 | 137 | 92 | 71 | 70 | ||
| No. true positive | 43 | 31 | 41 | 31 | 39 | 40 | ||
| No. false negative | 6 | 2 | 10 | 5 | 10 | 8 | ||
| No. true negative | 88 | 57 | 86 | 56 | 21 | 21 | ||
| No. false positive | 1 | 0 | 0 | 0 | 1 | 1 | ||
| Sensitivity, % (95% CI) | 87.8 (75.2–95.3) | 93.9 (79.8–99.3) | 80.4 (66.9–90.2) | 86.1 (70.5–95.3) | 79.6 (65.7–89.8) | 83.3 (69.8–92.5) | ||
| Specificity, % (95% CI) | 98.9 (93.9–100.0) | 100.0 (93.7–100.0)† | 100.0 (95.8–100.0) | 100.0 (93.6–100.0) | 95.5 (77.2–99.9) | 95.5 (77.2–99.9) | ||
*CCHF, Crimean-Congo hemorrhagic fever; IFA, immunofluorescent assay; qRT-PCR, quantitative reverse transcription PCR; LCD, low-cost, low-density.
†One-sided 95% CI.
