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Volume 18, Number 12—December 2012

Research

Diagnostic Assays for Crimean-Congo Hemorrhagic Fever

Jessica VanhomwegenComments to Author , Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, Helen Karlberg, Gülay Korukluoğlu, Miša Korva, Masoud Mardani, Ali Mirazimi, Mehrdad Mousavi, Anna Papa, Ana Saksida, Batool Sharifi-Mood, Persofoni Sidira, Katerina Tsergouli, Roman Wölfel, Hervé Zeller, and Philippe Dubois
Author affiliations: Author affiliations: Institut Pasteur, Paris, France (J. Vanhomwegen, P. Dubois); European Centre for Disease Control, Stockholm, Sweden (J. Vanhomwegen, H. Zeller); National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal (M.J. Alves); Faculty of Medicine, Ljubljana, Slovenia (T. Avšič Županc, M. Korva, A. Saksida); Institute of Public Health, Tirana, Albania (S. Bino); Pasteur Institute of Iran, Tehran, Iran (S. Chinikar); Swedish Institute for Infectious Disease Control, Solna, Sweden (H. Karlberg, A. Mirazimi, M. Mousavi); Refik Saydam National Public Health Agency, Ankara, Turkey (G. Korukluoğlu); Shahid Beheshti University of Medical Sciences, Tehran, Iran (M. Mardani, B. Sharifi-Mood); Aristotle University of Thessaloniki, Thessaloniki, Greece (A. Papa, P. Sidira, K. Tsergouli); and Bundeswehr Institute of Microbiology, Munich, Germany (R. Wölfel)

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Table 4

Overall performance of assays compared in study of CCHF diagnostic tools

Parameter IgM serology
IgG serology
Genome detection
ELISA IFA ELISA IFA qRT-PCR LCD array
No. samples tested 138 90 137 92 71 70
No. true positive 43 31 41 31 39 40
No. false negative 6 2 10 5 10 8
No. true negative 88 57 86 56 21 21
No. false positive 1 0 0 0 1 1
Sensitivity, % (95% CI) 87.8 (75.2–95.3) 93.9 (79.8–99.3) 80.4 (66.9–90.2) 86.1 (70.5–95.3) 79.6 (65.7–89.8) 83.3 (69.8–92.5)
Specificity, % (95% CI) 98.9 (93.9–100.0) 100.0 (93.7–100.0)† 100.0 (95.8–100.0) 100.0 (93.6–100.0) 95.5 (77.2–99.9) 95.5 (77.2–99.9)

*CCHF, Crimean-Congo hemorrhagic fever; IFA, immunofluorescent assay; qRT-PCR, quantitative reverse transcription PCR; LCD, low-cost, low-density.
†One-sided 95% CI.

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