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Volume 18, Number 12—December 2012

Letter

Hepatitis E Virus Genotype 3 in Shellfish, United Kingdom

Claire Crossan, Paul J. Baker, John Craft, Yasu Takeuchi, Harry R. Dalton, and Linda ScobieComments to Author 
Author affiliations: Author affiliations: Glasgow Caledonian University, Glasgow, Scotland, UK (C. Crossan, P.J. Baker, J. Craft, L. Scobie); University College London, London, UK (Y. Takeuchi); European Centre for Environment and Human Health, University of Exeter Medical School, Truro, UK (H.R. Dalton)

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Figure

Phylogenetic analysis of HEV open reading frame 2 sequences isolated from Mytilus spp. RNA was isolated from 50–100 mg of digestive gland or gill. Tissue was homogenized in 300 μL phosphate-buffered saline, and viral RNA was isolated by using a viral RNA kit (QIAGEN, Crawley, UK), and PCR was conducted by amplifying nucleotides 6332–6476 as described (8). The nucleotide sequences were aligned and bootstrapped, and phylogenetic neighbor-joining trees were constructed by using the ClustalW softwar

Figure. . Phylogenetic analysis of HEV open reading frame 2 sequences isolated from Mytilus spp. RNA was isolated from 50–100 mg of digestive gland or gill. Tissue was homogenized in 300 μL phosphate-buffered saline, and viral RNA was isolated by using a viral RNA kit (QIAGEN, Crawley, UK), and PCR was conducted by amplifying nucleotides 6332–6476 as described (8). The nucleotide sequences were aligned and bootstrapped, and phylogenetic neighbor-joining trees were constructed by using the ClustalW software (www.ebi.ac.uk/Tools/msa/clustalw2/). Phylogenetic trees were visualized by using FigTree (http://tree.bio.ed.ac.uk/software/figtree/). Bootstrap values >70% are indicated. Scale bar indicates nucleotide substitutions per site. Sample site codes: AB, Ardrossan Beach; LB, Lunderston Bay; ABN, Aberdeen; FB, Ferrybridge; YE, Ythan Estuary. Sequences: Sw, swine; hu, human (followed by country of origin). GenBank accession numbers for reference sequences: HEV genotype 1, B73218; HEV genotype 2, M74506; HEV genotype 3, CO31008; HEV genotype 4, C272108; huUK (KernowC1), HQ389543; HuUS, JN837481; swUK AF503512; huFrance, JN906974; swCanada, AY115488; swSpain, JQ522948; sw2Japan AB248521, huJapan AB161719.

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