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Volume 18, Number 2—February 2012

Letter

Leishmania infantum and Human Visceral Leishmaniasis, Argentina

Alejandra BarrioComments to Author , Cecilia M. Parodi, Fabricio Locatelli, María C. Mora, Miguel A. Basombrío, Masataka Korenaga, Yoshihisa Hashiguchi, María F. García Bustos, Alberto Gentile, and Jorge D. Marco
Author affiliations: Universidad Nacional de Salta, Salta, Argentina (A. Barrio, C.M. Parodi, M.C. Mora, M.A. Basombrío, M.F. García Bustos, J.D. Marco); Kochi Medical School, Kochi, Japan (F. Locatelli, M. Korenaga, Y. Hashiguchi); Ministerio de Salud Pública-Provincia de Salta, Salta (A. Gentile)

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Figure

Case of autochthonous human visceral leishmaniasis in a 44-year-old man, identified by parasitologic diagnosis and molecular detection of the causative species, Salta, Argentina. A) Leishmania amastigotes in a bone marrow smear. N, nucleus; K, kinetoplast; C, cytoplasm (Giemsa stained, original magnification ×1,000). B) Amplification by nested PCR of cytochrome b gene of Leishmania infantum. Arrow indicates amplified fragment of ≈850 bp. Lane B, blank control; lanes 1 and 2, patient bone marrow

Figure. Case of autochthonous human visceral leishmaniasis in a 44-year-old man, identified by parasitologic diagnosis and molecular detection of the causative species, Salta, Argentina. A) Leishmania amastigotes in a bone marrow smear. N, nucleus; K, kinetoplast; C, cytoplasm (Giemsa stained, original magnification ×1,000). B) Amplification by nested PCR of cytochrome b gene of Leishmania infantum. Arrow indicates amplified fragment of ≈850 bp. Lane B, blank control; lanes 1 and 2, patient bone marrow aspirate samples; lanes 3–6, samples from Leishmania spp.–negative persons; lane P, positive control; lane N, negative control; lane M, 100-bp molecular mass marker.

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