Leishmania infantum and Human Visceral Leishmaniasis, Argentina
Alejandra Barrio , Cecilia M. Parodi, Fabricio Locatelli, María C. Mora, Miguel A. Basombrío, Masataka Korenaga, Yoshihisa Hashiguchi, María F. García Bustos, Alberto Gentile, and Jorge D. Marco
Author affiliations: Universidad Nacional de Salta, Salta, Argentina (A. Barrio, C.M. Parodi, M.C. Mora, M.A. Basombrío, M.F. García Bustos, J.D. Marco); Kochi Medical School, Kochi, Japan (F. Locatelli, M. Korenaga, Y. Hashiguchi); Ministerio de Salud Pública-Provincia de Salta, Salta (A. Gentile)
Figure. Case of autochthonous human visceral leishmaniasis in a 44-year-old man, identified by parasitologic diagnosis and molecular detection of the causative species, Salta, Argentina. A) Leishmania amastigotes in a bone marrow smear. N, nucleus; K, kinetoplast; C, cytoplasm (Giemsa stained, original magnification ×1,000). B) Amplification by nested PCR of cytochrome b gene of Leishmania infantum. Arrow indicates amplified fragment of ≈850 bp. Lane B, blank control; lanes 1 and 2, patient bone marrow aspirate samples; lanes 3–6, samples from Leishmania spp.–negative persons; lane P, positive control; lane N, negative control; lane M, 100-bp molecular mass marker.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.