Volume 18, Number 2—February 2012
Research
Association of Human Bocavirus 1 Infection with Respiratory Disease in Childhood Follow-up Study, Finland
Mira Meriluoto, Lea Hedman, Laura Tanner, Ville Simell, Marjaana Mäkinen, Satu Simell, Juha Mykkänen, Jan Korpelainen, Olli Ruuskanen, Jorma Ilonen, Mikael Knip, Olli Simell, Klaus Hedman, and Maria Söderlund-Venermo
Table 3
Serologic and quantitative PCR results of consecutive serum samples from a representative child, showing all acute HBoV markers, Finland*
| Sample no. | Age at sample collection, y | Sampling interval, d | IgG absorbance | IgG interpretation† | IgM absorbance | IgM interpretation‡ | IgG avidity, %§ | qPCR, copies/mL |
|---|---|---|---|---|---|---|---|---|
| 1 | 0.33 | 120 | 0.061 | Neg | 0.015 | Neg | ||
| 2 | 0.99 | 235 | 0.010 | Neg | 0.014 | Neg | ||
| 3 | 1.25 | 96 | 0.029 | Neg | 0.025 | Neg | ||
| 4 | 1.52 | 96 | 0.032 | Neg | 0.023 | Neg | ||
| 5 | 1.77 | 90 | 0.032 | Neg | 0.021 | Neg | ||
| 6 | 2.02 | 89 | 0.017 | Neg | 0.022 | Neg | ||
| 7 | 2.52 | 180 | 0.019 | Neg | 0.028 | Neg | ||
| 8 | 2.97 | 165 | 0.014 | Neg | 0.028 | Neg | Neg | Neg |
| 9 | 3.44 | 167 | 1.536 | Pos | 0.730 | Pos | 2.8 | 7.67 × 104 |
| 10 | 3.93 | 178 | 2.883 | Pos | 0.023 | Neg | 60.8 | Neg |
| 11 | 4.46 | 190 | 3.412 | Pos | 0.035 | Neg | ||
| 12 | 4.91 | 163 | 3.111 | Pos | 0.022 | Neg | ||
| 13 | 5.45 | 193 | 3.754 | Pos | 0.033 | Neg | ||
| 14 | 5.96 | 185 | 3.228 | Pos | 0.020 | Neg | ||
| 15 | 7.07 | 398 | 3.183 | Pos | 0.030 | Neg | ||
| 16 | 7.52 | 163 | 3.102 | Pos | 0.026 | Neg | ||
| 17 | 8.07 | 198 | 3.450 | Pos | 0.027 | Neg | ||
| 18 | 8.53 | 165 | 3.149 | Pos | 0.016 | Neg | ||
| 19 | 9.07 | 195 | 3.252 | Pos | 0.024 | Neg | 49.2 |
*This child experienced an acute HBoV infection at 3 years of age (sample no. 9), evidenced by: IgG conversion, IgM, low IgG avidity and viremia (boldface). HBoV, human bocavirus; qPCR, quantitative PCR in serum; neg, negative; pos, positive; blank cells, not done.
†The cutoff absorbances for negative and positive IgG results were 0.154 (mean + 3 SD) and 0.188 (mean + 4 SD), respectively (13).
‡The cutoff absorbances for negative and positive IgM results were 0.136 (mean + 3 SD) and 0.167 (mean + 4 SD), respectively (13).
§The low- and high-avidity cutoff values: 15% and 25%, respectively (17).
