Volume 18, Number 7—July 2012
Dispatch
Role of Birds in Dispersal of Etiologic Agents of Tick-borne Zoonoses, Spain, 2009
Ana M. Palomar, Paula Santibáñez, David Mazuelas, Lidia Roncero, Sonia Santibáñez, Aránzazu Portillo, and José A. Oteo
Table 1
PCR primer pairs used in study of the role of birds in dispersal of etiologic agents of tick-borne zoonoses, Spain, 2009*
| Bacteria | Gene target | Primer name | Primer sequence, 5′ → 3′ | Amplified fragment, bp | Annealing temp., °C | Ref. |
|---|---|---|---|---|---|---|
| Anaplasma spp. | 16S rRNA, nested | ge3a | CACATGCAAGTCGAACGGATTATTC | 932 | 55 | (9) |
| ge10r | TTCCGTTAAGAAGGAT CTAATCTCC | |||||
| ge9f | AACGGATTATTCTTTATAGCTTGCT | 546 | 55 | (9) | ||
| ge2 | GGCAGTATTAAAAGCAGCTCCAGG | |||||
| msp | msp3F | CCAGCGTTTAGCAAGATAAGAG | 334 | 56 | (10) | |
| msp3R | GCCCAGTAACAACATCATAAGC | |||||
| Borrelia spp. | flaB, nested† | Outer 1 | AARGAATTGGCAGTTCAATC | 497 | 52 | (11) |
| Outer 2 | GCATTTTCWATTTTAGCAAGTGATG | |||||
| Inner 1 | ACATATTCAGATGCAGACAGAGGTTCTA | 389 | 55 | (11) | ||
| Inner 2 | GAAGGTGCTGTAGCAGGTGCTGGCTGT | |||||
| 5S-23S intergenic spacer, nested | 23SC1 | TAAGCTGACTAATACTAATTACCC | 380 | 52 | (12) | |
| 23SN1 | ACCATAGACTCTTATTACTTTGAC | |||||
| 5SCB | GAGAGTAGGTTATTGCCAGGG | 226 | 55 | (12) | ||
| 23SN2 | ACCATAGACTCTTATTACTTTGACCA | |||||
| Rickettsia spp. | ompA, seminested | Rr190.70p | ATGGCGAATATTTCTCCAAAA | 631 | 46 | (13,14) |
| Rr190.701n | GTTCCGTTAATGGCAGCATCT | |||||
| Rr190.70p | ATGGCGAATATTTCTCCAAAA | 532 | 48 | (14) | ||
| Rr190.602n | AGTGCAGCATTCGCTCCCCCT | |||||
| ompB, nested | rompB OF | GTAACCGGAAGTAATCGTTTCGTAA | 511 | 54 | (15) | |
| rompB OR | GCTTTATAACCAGCTAAACCACC | |||||
| rompB SFG IF | GTTTAATACGTGCTGCTAACCAA | 420 | 56 | (15) | ||
| rompB SFG/TG IR | GGTTTGGCCCATATACCATAAG | |||||
| gltA central region, nested | RpCS.877p | GGGGGCCTGCTCACGGCGG | 381 | 48 | (14) | |
| RpCS1258n | ATTGCAAAAAGTACAGTGAACA | |||||
| RpCS.896p | GGCTAATGAAGCAGTGATAA | 337 | 54 | (15) | ||
| RpCS.1233n | GCGACGGTATACCCATAGC |
*Temp., temperature; ref., reference; msp, p44 major surface protein gene; flaB, flagellin gene; ompB, 120-kDa genus common antigen gene; ompA, 190-kDa protein antigen gene; gltA, citrate synthase gene.
†R = A/G; W = A/T.
