Lasse Dam Rasmussen, Birgit Kristensen, Carsten Kirkeby, Thomas Bruun Rasmussen, Graham J. Belsham, René Bødker, and Anette Bøtner
Author affiliations: Technical University of Denmark, Kalvehave, Denmark (L.D. Rasmussen, T.B. Rasmussen, G.J. Belsham, A. Bøtner); Technical University of Denmark, Copenhagen, Denmark (B. Kristensen, C. Kirkeby, R. Bødker)
Figure. . . RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse transcription quantitative PCRs (RT-qPCRs) for the Schmallenberg virus large segment, and the products were analyzed by agarose gel electrophoresis. Lanes 1–8, C. obsoletus group midge pools 1–8; lanes 9–10; negative and positive controls, respectively. Numbers below lanes are cycle threshold values from RT-qPCRs; –, no value. M, size marker. Amplicons (145 bp) from positive pools were extracted and sequenced.
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