Volume 18, Number 9—September 2012
Dispatch
Rapid Detection of Carbapenemase-producing Enterobacteriaceae
Patrice Nordmann
, Laurent Poirel, and Laurent Dortet
, Laurent Poirel, and Laurent DortetTable 1
Carbapenemase-producing clinical enterobacterial isolates subjected to the Carba NP test*
| Ambler class, carbapenemase type | Species | β-Lactamase | No. | MIC range, mg/L |
Carba NP test result | ||
|---|---|---|---|---|---|---|---|
| IMP | ERT | MER | |||||
| Class A | |||||||
| KPC-type |
Klebsiella pneumoniae | KPC-2 | 27 | 0.5–>32 | 4–>32 | 1–>32 | + |
| KPC-3 | 3 | 0.5–8 | 4–>32 | 1–8 | + | ||
| Klebsiella ozaenae | KPC-3 | 1 | >32 | >32 | 2 | + | |
| Escherichia coli | KPC-2 | 5 | 0.5–4 | 0.5>32 | 0.5–2 | + | |
| Enterobacter cloacae | KPC-2 | 7 | 1–24 | 1.5–32 | 0.75–16 | + | |
| Enterobacter aerogenes | KPC-2 | 1 | 8 | >32 | 8 | + | |
| Citrobacter freundii | KPC-2 | 2 | 8–>32 | 1.5–>32 | 1.5–3 | + | |
| Serratia marcescens | KPC-2 | 2 | >32 | >32 | >32 | + | |
| Salmonella spp. |
KPC-2 |
1 |
4 |
1 |
0.25 |
+ |
|
| NMC-A |
E. cloacae |
NMC-A |
1 |
16 |
>32 |
16 |
+ |
| SME-type |
S. marcescens |
SME-1 | 1 | 32 | 4 | 12 | + |
| SME-2 |
1 |
32 |
4 |
12 |
+ |
||
| GES-type |
E. cloacae |
GES-5 |
1 |
>32 |
>32 |
>32 |
+ |
| IMI-type |
Enterobacter asburiae |
IMI-2 |
1 |
>32 |
>32 |
>32 |
+ |
| Class B | |||||||
| NDM-type | K. pneumoniae | NDM-1 | 16 | 0.5–>32 | 2–>32 | 1–>32 | + |
| NDM-4 | 1 | >32 | >32 | >32 | + | ||
| E. coli | NDM-1 | 7 | 1–16 | 3–>32 | 1–16 | + | |
| E. cloacae | NDM-1 | 1 | 2 | 16 | 2 | + | |
| C. freundii | NDM-1 | 1 | >32 | >32 | >32 | + | |
| Providencia stuartii | NDM-1 | 1 | 12 | 0.38 | 1.5 | + | |
| Proteus rettgeri |
NDM-1 |
1 |
3 |
0.5 |
1.5 |
+ |
|
| VIM-type |
K. pneumoniae | VIM-1 | 15 | 0.5–>32 | 0.5–>32 | 0.38–>32 | + |
| VIM-19 | 1 | 8 | 16 | 4 | + | ||
| E. coli | VIM-1 | 2 | 1.5–3 | 0.38–1.5 | 0.5–1 | + | |
| VIM-2 | 2 | 2–4 | 0.5–1.5 | 0.38–0.5 | + | ||
| VIM-19 | 1 | 8 | 16 | 4 | + | ||
| E. cloacae | VIM-1 | 4 | 1–>32 | 0.38 to >32 | 0.5–>32 | + | |
| S. marcescens |
VIM-2 |
1 |
>32 |
>32 |
>32 |
+ |
|
| IMP-type |
K. pneumoniae | IMP-1 | 5 | 0.5–8 | 2–4 | 1–8 | + |
| IMP-8 | 2 | 0.5–1 | 0.5–1 | 0.5 | + | ||
| E. coli | IMP-1 | 2 | 0.5 | 3–4 | 0.5–1 | + | |
| IMP-8 | 1 | 6 | 8 | 3 | + | ||
| E. cloacae | IMP-1 | 12 | 8–>32 | >32 | 2–>32 | + | |
| IMP-8 | 2 | 0.75–1.5 | 0.5–1 | 0.5–1 | + | ||
| S. marcescens | IMP-1 | 2 | 8–>32 | >32 | 2–>32 | + | |
| IMP-11 |
1 |
8 |
>32 |
2 |
+ |
||
| Class D | |||||||
| OXA-48 type | K. pneumoniae | OXA-48 | 15 | 0.38–>32 | 0.38–>32 | 0.38–>32 | + |
| OXA-181 | 2 | 0.5-1 | 2–4 | 0.5–1 | + | ||
| E. coli | OXA-48 | 6 | 0.38–3 | 0.5–16 | 0.12–1 | + | |
| E. cloacae | OXA-48 | 3 | 0.5–1 | 0.5–16 | 0.5–1.5 | + | |
| P. rettgeri | OXA-181 | 1 | 8 | 1 | 2 | + | |
*IMP, imipenem; ERT, ertapenem; MER, meropenem; KPC, K. pneumoniae carbapenemase; NMC-A, non–metallo-enzyme carbapenemase; SME, S. marscescens enzyme; GES, Guiana extended-spectrum β-lactamase; IMI, imipenem-hydrolysing β-lactamase; NDM, New Delhi metallo-β-lactamase; VIM, Verona integron–encoded metallo-β-lactamase; IMP, imipenemase; OXA-48, oxacillinase-48.
