Volume 18, Number 9—September 2012
Dispatch
Rapid Detection of Carbapenemase-producing Enterobacteriaceae
Patrice Nordmann
, Laurent Poirel, and Laurent Dortet
, Laurent Poirel, and Laurent DortetTable 2
Non–carbapenemase-producing clinical enterobacterial isolates subjected to the Carba NP test*
| β-Lactamase type, species | β-Lactamase | No. | MIC, mg/L |
Carba NP test result | ||
|---|---|---|---|---|---|---|
| IMP | ERT | MER | ||||
| ESBLs | ||||||
| Klebsiella pneumoniae | CTX-M-3 | 1 | 0.12 | 0.12 | 0.12 | – |
| CTX-M-14 | 1 | 0.12 | 0.12 | 0.12 | – | |
| CTX-M-15 | 3 | 0.12 | 0.12 | 0.12 | – | |
| Escherichia coli | CTX-M-1 | 1 | 0.12 | 0.12 | 0.12 | – |
| CTX-M-3 | 1 | 0.12 | 0.12 | 0.12 | – | |
| CTX-M-14 | 2 | 0.12 | 0.12 | 0.12 | – | |
| CTX-M-15 | 2 | 0.12 | 0.12 | 0.12 | – | |
| VEB-1 | 1 | 0.12–0.25 | 0.12 | 0.12 | – | |
| Enterobacter cloacae | CTX-M-15 | 3 | 0.12 | 0.12 | 0.12 | – |
| VEB-1 |
1 |
0.12 |
0.12 |
0.12 |
– |
|
| Plasmid-mediated AmpC or chromosomal AmpC + decreased membrane permeability | ||||||
| K. pneumoniae | DHA-1 | 1 | >32 | >32 | >32 | – |
| DHA-2 | 1 | 0.12 | 0.5 | 0.12 | – | |
| E. coli | Extended-spectrum cephalosporinase | 1 | 0.12 | 0.12 | 0.12 | – |
| CMY-2 | 1 | 0.12 | 0.12 | 0.12 | – | |
| CMY-10 | 1 | 0.12 | 0.38 | 0.12 | – | |
| DHA-1 | 1 | 0.12 | 0.12 | 0.12 | – | |
| ACC-1 | 1 | 0.12 | 0.12 | 0.12 | – | |
| Overexpressed cephalosporinase | 1 | 16 | >32 | 2 | – | |
| Proteus mirabilis | ACC-1 | 1 | 0.25 | 0.12 | 0.12 | – |
| E. cloacae | Overexpressed cephalosporinase | 7 | 0.12–16 | 1–>32 | 0.12–>32 | – |
| Enterobacter aerogenes | Overexpressed cephalosporinase | 1 | 1 | 4 | 0.75 | – |
| Morganella morganii |
Overexpressed cephalosporinase |
2 |
1.5–2 |
0.12 |
0.5 |
– |
| ESBL + decreased membrane permeability | ||||||
| K. pneumoniae | CTX-M-15 | 8 | 0.25–8 | 1–>32 | 1–>32 | – |
| SHV-28 | 1 | 1 | 4 | 1 | – | |
| SHV-2a | 1 | 0.25 | 2 | 0.38 | – | |
| Enterobacter sakazakii | CTX-M-15 | 1 | 0.25 | 1.5 | 0.25 | – |
| Citrobacter freundii | TEM-3 | 1 | 1 | 8 | 1 | – |
*IMP, imipenem; ERT, ertapenem; MER, meropenem; ESBLs, extended-spectrum β-lactamases.
