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Volume 19, Number 1—January 2013


Schmallenberg Virus in Culicoides spp. Biting Midges, the Netherlands, 2011

Armin R.W. ElbersComments to Author , Rudy Meiswinkel, Erik van Weezep, Marianne M. Sloet van Oldruitenborgh-Oosterbaan, and Engbert A. Kooi
Author affiliations: Central Veterinary Institute, Lelystad, the Netherlands (A.R.W. Elbers, E. van Weezep, E.A. Kooi); Rocca di Cave, Rome, Italy (R. Meiswinkel); and Utrecht University, Utrecht, the Netherlands (M.M. Sloet van Oldruitenborgh-Oosterbaan)

Main Article

Table 2

Ct values of Schmallenberg virus–positive Culicoides spp. biting midges collected August–September 2011, the Netherlands*

Pool no. Pooled heads
Individual abdomens
Species identification by morphologic examination Ct value
Species identification by DNA sequencing Ct value
First test Second test First test Second test
95-D C. chiopterus 27.88 NA C. chiopterus 24.59 NA
501 C. chiopterus 35.36 34.98 C. chiopterus 36.45 35.07
9-A C. obsoletus complex 30.44 NA C. scoticus 24.75 NA
18-C C. obsoletus complex 28.24 NA C. scoticus 24.95 NA
32-B C. obsoletus complex 21.84 NA C. scoticus 18.32 NA
259 C. obsoletus complex 19.60 NA C. scoticus 18.16 NA
275 C. obsoletus complex 20.72 NA C. scoticus 20.39 NA
276 C. obsoletus complex 36.02 36.78 C. scoticus 36.68 NA
293 C. obsoletus complex 20.43 NA No reliable sequence 19.95 NA
294 C. obsoletus complex 24.60 NA C. obsoletus sensu stricto 20.06 NA
368 C. obsoletus complex 25.21 NA C. scoticus 21.80 NA
385 C. obsoletus complex 20.67 NA C. scoticus 20.25 NA
405 C. obsoletus complex 23.38 NA C. scoticus 21.64 NA
434† C. obsoletus complex 23.68 NA C. scoticus 23.10 NA

*Ct values determined by reverse transcription PCR. All midges were collected from cattle in the Ermelo municipality except no. 95-D, which was collected from sheep in the Midden-Drente municipality. Ct, cycle threshold; NA, not applicable.
†Two abdomens from this pool were positive; Ct values for the second abdomen were 35.75 and 35.37.

Main Article