Emerging Infectious Disease ISSN: 1080-6059

Novel Polyomavirus associated with Brain Tumors in Free-Ranging Raccoons, Western United States

Florante N. Dela Cruz, Federico Giannitti, Linlin Li, Leslie W. Woods, Luis Del Valle, Eric Delwart, and Patricia A. Pesavento

Author affiliations: Author affiliations: University of California, Davis, Davis, California, USA (F.N. Dela Cruz, Jr., F. Giannitti, L.W. Woods, P.A. Pesavento); Blood Systems Research Institute, San Francisco, California, USA (L. Li, E. Delwart); Louisiana State University, New Orleans, Louisiana, USA (L. Del Valle); University of California, San Francisco, San Francisco (E. Delwart)

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Figure 4

Partial genome sequences (ranging from 2,998 bp in raccoon polyomavirus 6 [RacPyV6] to 4,667 bp in RacPyV9) were obtained from 4 raccoons that had undergone prolonged storage (RacPyV9) or were available only as formalin-fixed, paraffin-embedded tissue (dashed line, RacPyVs 1, 6, and 7). Gaps in the sequences correspond to regions where amplification reactions failed. The genomes of RacPyVs 2, 3, 4, 5, 8, and 10 were sequenced in their entirety by using a primer walking method to complete the Rac

Figure 4. . Partial genome sequences (ranging from 2,998 bp in raccoon polyomavirus 6 [RacPyV6] to 4,667 bp in RacPyV9) were obtained from 4 raccoons that either had undergone prolonged storage (RacPyV9) or were available only as formalin-fixed, paraffin-embedded tissue (dashed lines, RacPyVs 1, 6, and 7). Gaps in the sequences correspond to regions where amplification reactions failed. The genomes of RacPyVs 2, 3, 4, 5, 8, and 10 were sequenced in their entirety by using a primer walking method to complete the RacPyV circular genome. Open circles represent noncoding mutations; the closed circle represents a coding mutation. Horizontal bars indicate deletions. LT-Ag, large T-antigen; VP, viral protein; NCRR, noncoding regulatory region.

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