Novel Polyomavirus associated with Brain Tumors in Free-Ranging Raccoons, Western United States
Florante N. Dela Cruz, Federico Giannitti, Linlin Li, Leslie W. Woods, Luis Del Valle, Eric Delwart, and Patricia A. Pesavento
Author affiliations: Author affiliations: University of California, Davis, Davis, California, USA (F.N. Dela Cruz, Jr., F. Giannitti, L.W. Woods, P.A. Pesavento); Blood Systems Research Institute, San Francisco, California, USA (L. Li, E. Delwart); Louisiana State University, New Orleans, Louisiana, USA (L. Del Valle); University of California, San Francisco, San Francisco (E. Delwart)
Figure 6. . Expression of large T-antigen (LT-Ag) and p53 within a subset of tumors. A) Control, immunohistochemical analysis of frontal lobe of normal raccoon brain tissue. Astrocytes in this image and throughout the section were not immunoreactive for LT-Ag. Original magnification ×40. B, C) Immunohistochemical analysis for raccoon no. 2 (Rac2) and Rac3. LT-Ag is expressed within the nuclei of a subset of neoplastic astrocytes in 2 independent tumors. Original magnification ×40. D) Immunohistochemical analysis for Rac3. p53 is present within the nuclei of a subset of tumor cells. Original magnification ×40. Scale bars for panels A–D = 100 μm. E–G) Immunofluorescence of Rac4. p53 co-localizes with LT-Ag within nuclei of neoplastic cells. Original magnification ×60.
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