Figure 2. . . Unrooted neighbor-joining tree computed in MEGA 5.0 (10) for multivirulence locus sequence typing data based on sequencing of 6 virulence genes, prfA, inlB, inlC, dal, clpP, and lisR (3) obtained for the 93 Listeria monocytogenes isolates compared in this study. Nine cantaloupe-associated outbreak isolates were from the Centers for Disease Control and Prevention (CDC) (GenBank accession nos. JQ407055–JQ407078) and the Food and Drug Administration (FDA) (JX141237–JX141275), 23 isolates from Knabel et al., 2012 (4), 29 isolates from Chen et al., 2007 (3), 29 from Berrang et al., 2005 and 2010 (7,8) (JQ946653–JQ946836), and 3 other isolates for which sequence data were available in GenBank. Squares indicate reference strains representing the 5 currently known L. monocytogenes epidemic clones. Underlined text indicates CDC and FDA isolates associated with the isolates from the 2011 cantaloupe-associated outbreak. Circles indicate strains classified as epidemic clone (EC)VI (indicated in blue). Triangles indicate strains classified as ECVII (indicated in red). Diamond indicates the novel outbreak strain (indicated in green).