Volume 19, Number 4—April 2013
Hepatitis E Virus Genotype 3 Strains in Domestic Pigs, Cameroon
To the Editor: Hepatitis E virus (HEV) is a positive-stranded, non-enveloped RNA virus of the family Hepeviridae that is considered to be the main causative agent of enterically transmitted acute hepatitis (1). HEV is classified into 4 genotypes (1). HEV genotypes 1 and 2 cause large waterborne epidemics of acute hepatitis in developing countries, especially in Africa and Asia (1). In contrast, HEV genotypes 3 and 4 are increasingly identified as causative agents of acute viral hepatitis in industrialized countries (1). Genotypes 1 and 2 are found only in humans, whereas genotypes 3 and 4 are associated with food-borne zoonotic transmission from domestic pigs, wild boar, and deer (1).
In addition to these 4 genotypes, HEV-related viruses were detected in avian, rodent, and bat hosts, which formed novel genera within the family Hepeviridae (2). In Africa, HEV genotype 1 and 2 strains have been identified during HEV epidemics (3–5). An HEV genotype 3 strain was detected in 1 of 40 fecal samples from domestic pigs in Kinshasa, Democratic Republic of the Congo, and it was suggested that this strain was imported from Belgium to the Democratic Republic of the Congo by animal trade (6). Therefore, we investigated whether HEV strains of genotype 3 or 4 are circulating among domestic pigs in Cameroon.
During February–March 2012, a total of 345 liver samples were collected from domestic pigs (age range 6 months–3 years) in abattoirs in Douala and Yaoundé, Cameroon, and in slaughter slaps (areas) in Bamenda, Cameroon. Pigs were mainly of the local breed. In addition, pigs originating from extensive cross-breeding (local X landrace and local X Duroc) were sampled. Liver samples were collected during post-mortem inspection.
Viral RNA was extracted from liver samples by using the RTP DNA/RNA Virus Mini Kit II (STRATEC Molecular, Berlin, Germany) according to the manufacturer’s instructions. Extracted RNA was analyzed for HEV RNA by using 2 nested reverse transcription PCRs (RT-PCRs) specific for open reading frame 1 (OFR 1) and ORF 2 of HEV (7,8). Nested RT-PCRs and direct sequencing of amplicons were performed as described (9). RNA of HEV strain Hamburg-HB (GenBank accession no. JN986840) was used as a positive control for nested RT-PCRs.
HEV RNA was detected in 2 samples from female pigs in Yaoundé (2/139) and 1 sample from a male pig in Bamenda (1/39). All 167 samples from Douala were negative for HEV RNA. The sample from Bamenda showed a positive result for the nested RT-PCR specific for HEV ORF 1. Genetic distances calculated with partial nucleotide sequences of ORF 1 (280 nt) and ORF 2 (373 nt) between strain Yaounde56 and the most closely related HEV genotype 3 strains from Japan (JSWINE150-Aom04R; GenBank accession no. AB221520) and Mongolia (swMN06-A1354; GenBank accession no. AB290105) were 90% and 91%, respectively.
At the amino acid level, the partial RNA-dependent RNA polymerase sequence (ORF 1) and the partial capsid protein sequence (ORF 2) of strain Yaounde56 were identical to those of HEV genotype 3 strains HEV/Gt3/HSD40/2009 (GenBank accession no. AFO71833) from Germany and swJ12–1 (GenBank accession no. BAC66273) from Japan. Thus, all mutations were silent.
In agreement with distance analysis, phylogenetic reconstruction using partial nucleotide sequences of ORF 2 (278 nt) showed a close relationship of strains Yaounde56 and Yaounde94 with HEV genotype 3 strains (Figure). However, the HEV strains from Cameroon do not cluster with the classified HEV genotype 3 subtype reference strains (10) in the phylogenetic tree (Figure). These strains cluster within a clade of subtype undefined strains and are most closely related to strain swMN06-A1354 from Mongolia (Figure).
Because the pig production cycle is shorter than that for cattle, pig production is a major economic activity in Cameroon. Most pigs in Cameroon are local raised, and extensive cross-breeding is used. The infection rate of pigs with HEV genotype 3 strains from Cameroon is lower than that of pigs from Europe. Thus, HEV genotype 3 seems to have an extensive distribution that includes Africa. Future studies should investigate how HEV genotype 3 strains contribute to sporadic HEV cases in Cameroon.
Author affiliations: Fundação Oswaldo Cruz, Rio de Janeiro, Brazil (de Paula VS); Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (de Paula VS, M. Wiele, J. Schmidt-Chanasit); Centre Pasteur, Yaoundé, Cameroon (A.H. Mbunkah); Institute of Agricultural Research for Development, Ngaoundere, Cameroon (A.M. Daniel, M.T. Kingsley)
This study was supported by Coordenacão de Aperfeicoamento de Pessoal de Nível Superior.
- Kamar N, Bendall R, Legrand-Abravanel F, Xia NS, Ijaz S, Izopet J, Hepatitis E.Lancet. 2012;379:2477–88 and.
- Drexler JF, Seelen A, Corman VM, Fumie Tateno A, Cottontail V, Melim Zerbinati R, Bats worldwide carry hepatitis E virus-related viruses that form a putative novel genus within the family Hepeviridae.J Virol. 2012;86:9134–47 and.
- He J, Binn LN, Tsarev SA, Hayes CG, Frean JA, Isaacson M, Molecular characterization of a hepatitis E virus isolate from Namibia.J Biomed Sci. 2000;7:334–8 and.
- van Cuyck H, Juge F, Roques P. Phylogenetic analysis of the first complete hepatitis E virus (HEV) genome from Africa.FEMS Immunol Med Microbiol. 2003;39:133–9 and.
- Nicand E, Armstrong GL, Enouf V, Guthmann JP, Guerin JP, Caron M, Genetic heterogeneity of hepatitis E virus in Darfur, Sudan, and neighboring Chad.J Med Virol. 2005;77:519–21 and.
- Kaba M, Colson P, Musongela JP, Tshilolo L, Davoust B. Detection of hepatitis E virus of genotype 3 in a farm pig in Kinshasa (Democratic Republic of the Congo).Infect Genet Evol. 2010;10:154–7 and.
- Johne R, Plenge-Bönig A, Hess M, Ulrich RG, Reetz J, Schielke A. Detection of a novel hepatitis E-like virus in faeces of wildrats using a nested broad spectrum RT-PCR.J Gen Virol. 2010;91:750–8 and.
- Mizuo H, Suzuki K, Takikawa Y, Sugai Y, Tokita H, Akahane Y, Polyphyletic strains of hepatitis E virus are responsible for sporadic cases of acute hepatitis in Japan.J Clin Microbiol. 2002;40:3209–18 and.
- Pfefferle S, Frickmann H, Gabriel M, Schmitz N, Günther S, Schmidt-Chanasit J. Fatal course of an autochthonous hepatitis E virus infection in a patient with leukemia in Germany.Infection. 2012;40:451–4 and.
- Lu L, Li C, Hagedorn CH. Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis.Rev Med Virol. 2006;16:5–36 and.
- Figure. . . . . . Phylogenetic analysis of hepatitis E virus (HEV) strains, Cameroon. The Bayesian phylogenetic tree was constructed by using partial nucleotide sequence of open reading frame 2...
Suggested citation for this article: de Paula VS, Wiele M, Mbunkah AH, Daniel AM, Kingsley MT, Schmidt-Chanasit J. Hepatitis E virus genotype 3 strains in domestic pigs, Cameroon [letter]. Emerg Infect Dis [Internet]. 2013 Apr [date cited]. http://dx.doi.org/10.3201/eid1904.121364
1These authors contributed equally to this article.
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Jonas Schmidt-Chanasit, Bernhard Nocht Institute for Tropical Medicine, World Health Organization Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany
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