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Volume 19, Number 7—July 2013

Dispatch

Asynchronous Onset of Clinical Disease in BSE-Infected Macaques

Judith Montag1, Walter Schulz-Schaeffer, Annette Schrod, Gerhard Hunsmann, and Dirk MotzkusComments to Author 
Author affiliations: German Primate Center, Göttingen, Germany (J. Montag, A. Schrod, G. Hunsmann, D. Motzkus); University of Göttingen, Göttingen (W. Schulz-Schaeffer)

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Figure 2

PrPSc profile of macaque-adapted BSE in comparison to human CJD. Brain homogenates from human sCJD type 1, sCJD type 2, vCJD, and BSE-infected macaques were subjected to PK treatment, separated on 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and blotted onto nitrocellulose membranes. A) PrPSc for human and macaque brain was detected with the widely used monoclonal antibody 3F4 or with 11C6. B) Glycoform ratio of sCJD type 2, vCJD, and macaque-adapted BSE. The relative signal in

Figure 2. . . PrPSc profile of macaque-adapted BSE in comparison to human CJD. Brain homogenates from human sCJD type 1, sCJD type 2, vCJD, and BSE-infected macaques were subjected to PK treatment, separated on 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and blotted onto nitrocellulose membranes. A) PrPSc for human and macaque brain was detected with the widely used monoclonal antibody 3F4 or with 11C6. B) Glycoform ratio of sCJD type 2, vCJD, and macaque-adapted BSE. The relative signal intensities of the diglycosylated, monoglycosylated, and nongylcosylated isoforms were determined densitometrically and normalized to the band of the nongylcosylated isoform. PrPSc, prion protein isoform; BSE, bovine spongiform encephalopathy; CJD, Creutzfeldt-Jakob disease; sCJD, sporadic CJD; vCJD, variant CJD; PK, proteinase K.

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1Current affiliation: Hannover Medical School, Hannover, Germany.

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