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Volume 19, Number 8—August 2013

Research

Comparison of 2 Assays for Diagnosing Rotavirus and Evaluating Vaccine Effectiveness in Children with Gastroenteritis

Jacqueline E. TateComments to Author , Slavica Mijatovic-Rustempasic, Ka Ian Tam, Freda C. Lyde, Daniel C. Payne, Peter Szilagyi, Kathryn Edwards, Mary Allen Staat, Geoffrey A. Weinberg, Caroline B. Hall, James Chappell, Monica McNeal, Jon R. Gentsch, Michael D. Bowen, and Umesh D. Parashar
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J.E. Tate, S. Mijatovic-Rustempasic, K.I. Tam, F.C. Lyde, D.C. Payne, J.R. Gentsch, M.D. Bowen, U.D. Parashar); University of Rochester School of Medicine and Dentistry, Rochester, New York, USA (P. Szilagyi, G.A. Weinberg, C.B. Hall); Vanderbilt University Medical Center, Nashville, Tennessee, USA (K. Edwards, J. Chappell); Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA (M.A. Staat, M. McNeal)

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Figure 2

Frequency distribution of Ct values for specimens in which rotavirus was detected by qRT-PCR, 3 New Vaccine Surveillance Network sites (USA), October 2008–October 2009. For 1 (1%) acute gastroenteritis EIA+ specimen, 425 (87%) acute gastroenteritis EIA− specimens, and 476 (95%) healthy control specimens, no virus was detected by qRT-PCR. Ct, cycle threshold; qRT-PCR, semiquantitative reverse transcription PCR; EIA, enzyme immunoassay; +, positive; −, negative. Black bars indicate acute gastroent

Figure 2. . . . Frequency distribution of Ct values for specimens in which rotavirus was detected by qRT-PCR, 3 New Vaccine Surveillance Network sites (USA), October 2008–October 2009. For 1 (1%) acute gastroenteritis EIA+ specimen, 425 (87%) acute gastroenteritis EIA− specimens, and 476 (95%) healthy control specimens, no virus was detected by qRT-PCR. Ct, cycle threshold; qRT-PCR, semiquantitative reverse transcription PCR; EIA, enzyme immunoassay; +, positive; −, negative. Black bars indicate acute gastroenteritis patients with EIA+ specimens, n = 157; gray bars indicate acute gastroenteritis patients with EIA− specimens, n = 65; white bars indicate healthy controls, n = 24.

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