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Volume 21, Number 1—January 2015
Letter

Serologic Assessment of Possibility for MERS-CoV Infection in Equids

Benjamin Meyer, Ignacio García-Bocanegra, Ulrich Wernery, Renate Wernery, Andrea Sieberg, Marcel A. Müller, Ana Maria Bispo de Filippis, Sung Sup ParkComments to Author , and Isabella Eckerle
Author affiliations: University of Bonn Medical Centre, Bonn, Germany (B. Meyer, A. Sieberg, M.A. Müller, C. Drosten, J.F. Drexler, I. Eckerle); Universidad de Córdoba, Córdoba, Spain (I. García-Bocanegra); Central Veterinary Research Laboratory, Dubai, United Arab Emirates (U. Wernery, R. Wernery)

Main Article

Figure

Analysis of the replication of Middle East respiratory syndrome coronavirus (MERS-CoV) in primary horse kidney cell lines and origin of equine serum samples. A, B) Cells were seeded at densities of 2 × 105 cells/mL and infected in triplicate with a multiplicity of infection of 0.5 infectious MERS-CoV units/cell. After incubation for 1 h, cells were washed twice and supernatants were harvested at 0, 20, and 40 h postinfection (hpi). The replication level is given as the log of the genome equivale

Figure. Analysis of the replication of Middle East respiratory syndrome coronavirus (MERS-CoV) in primary horse kidney cell lines and origin of equine serum samples. A, B) Cells were seeded at densities of 2 × 105 cells/mL and infected in triplicate with a multiplicity of infection of 0.5 infectious MERS-CoV units/cell. After incubation for 1 h, cells were washed twice and supernatants were harvested at 0, 20, and 40 h postinfection (hpi). The replication level is given as the log of the genome equivalents (A) or as PFUs (B). Error bars indicate ranges; PF-N and PFN-R indicate the 2 horse cell lines; VeroB4 is an interferon-deficient primate cell line. C) Distribution of optical density (OD) values (450 nm) of equine serum samples originating from Spain or the United Arab Emirates (UAE).

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