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Volume 21, Number 12—December 2015
Research

High Prevalence of Intermediate Leptospira spp. DNA in Febrile Humans from Urban and Rural Ecuador

Jorge Chiriboga, Verónica Barragan, Gabriela Arroyo, Andrea Sosa, Dawn N. Birdsell, Karool España, Ana Mora, Emilia Espín, María Eugenia Mejía, Melba Morales, Carmina Pinargote, Manuel Gonzalez, Rudy Hartskeerl, Paul Keim, Gustavo Bretas, Joseph N.S. Eisenberg, and Gabriel TruebaComments to Author 
Author affiliations: Microbiology Institute, Universidad San Francisco de Quito, Campus Cumbaya, Quito, Ecuador (J. Chiriboga, V. Barragan, G. Arroyo, A. Sosa, E. Espín, M.E. Mejía, G. Trueba); Northern Arizona University, Flagstaff, Arizona, USA (D.N. Birdsell, P. Keim); Instituto Nacional de Salud Pública e Investigación, Portoviejo, Ecuador (K. España, A. Mora, M. Morales, C. Pinargote, M. Gonzalez); Ministerio de Salud Pública, Portoviejo (M. Morales); Royal Tropical Institute (KIT), Amsterdam, the Netherlands (R. Hartskeerl); Organización Panamericana de la Salud OPS, Guayaquil, Ecuador (G. Bretas); University of Michigan, Ann Arbor, Michigan, USA (J.N.S. Eisenberg)

Main Article

Table 1

PCR results by using primer Inter- R combined with primers A and C*

Leptospira spp. Strain PCR result
Intermediate
L. broomii 5399 +
L. fainei BUT 6 +
L.inadai 10 +
L. wolffii Korat-H2T +
L. licerasiae
 VAR010
 +
Pathogenic
L. interrogans Pomona −
L. kirschneri Kambale −
L. borgpetersenii MUS 127 −
L. noguchii M7 −
L. alexanderi A85 −
L. santarosai CZ 390 −
L. weilii
 Sarmin
 −
Saprophytic
L. vanthielii WazHolland −
L. biflexa Patoc I −
L.meyeri ICF −
L. wolbachii CDC −
L. kmetyi Bejo-Iso9T −

*Only intermediate Leptospira species were amplified by using Inter-R specific primer. +, positive; –, negative.

Main Article

Page created: November 16, 2015
Page updated: November 16, 2015
Page reviewed: November 16, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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