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Volume 22, Number 11—November 2016
Research

Reassortant Eurasian Avian-Like Influenza A(H1N1) Virus from a Severely Ill Child, Hunan Province, China, 2015

Wenfei Zhu1, Hong Zhang1, Xingyu Xiang1, Lili Zhong, Lei Yang, Junfeng Guo, Yiran Xie, Fangcai Li, Zhihong Deng, Hong Feng, Yiwei Huang, Shixiong Hu, Xin Xu, Xiaohui Zou, Xiaodan Li, Tian Bai, Yongkun Chen, Zi Li, Junhua LiComments to Author , and Yuelong ShuComments to Author 
Author affiliations: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China (W. Zhu, L. Yang, J. Guo, Y. Xie, X. Zou, X. Li, T. Bai, Y. Chen, Z. Li, Y. Shu); Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing (W. Zhu, L. Yang, J. Guo, Y. Xie, X. Zou, X. Li, T. Bai, Y. Chen, Z. Li, Y. Shu); Hunan Provincial Center for Disease Control and Prevention, Changsha, China (H. Zhang, X. Xiang, F. Li, Z. Deng, Y. Huang, S. Hu, J. Li); Hunan Provincial People’s Hospital, Changsha (L. Zhong); Liuyang Center for Disease Control and Prevention, Liuyang, China (H. Feng, X. Xu)

Main Article

Table 1

Seroconversion in C57BL/6 mice inoculated with JS/1/11 EA-H1N1 and HuN EA-H1N1 viruses*

Virus and dose, log10 TCID50/50 μL
 Hemagglutination inhibition titer†
 MID50, log10 TCID50‡
JS/1/11 EA-H1N1 virus antigen
HuN EA-H1N1 virus antigen
Mouse 1
 Mouse 2
 Mouse 3
 Mouse 4
 Mouse 5
 Mouse 1
 Mouse 2
 Mouse 3
 Mouse 4
 Mouse 5
JS/1/11 EA-H1N1 4.7
6 40 20 >80 40 40 >80 40 >80 40 40
5 <10 40 20 20 40 10 >80 20 40 >80
4 10 10 <10 <10 <10 20 <10 <10 10 <10
3 <10 <10 <10 <10 <10 <10 <10 <10 <10 <10
2 <10 <10 <10 <10 <10 <10 <10 <10 <10 <10
1
 <10
 <10
 <10
 <10
 <10
<10
 <10
 <10
 <10
 <10
HuN EA-H1N1 2.9
6 ND ND ND ND ND ND ND ND ND ND
5 ND ND ND ND ND ND ND ND ND ND
4 >80 40 >80 >80 <10 >80 >80 >80 >80 <10
3 >80 40 40 <10 20 >80 >80 80 <10 40
2 <10 <10 <10 <10 <10 <10 <10 <10 <10 <10
1
 <10
 <10
 <10
 <10
 <10
<10
 <10
 <10
 <10
 10
PBS <10 <10 <10 <10 <10 <10 <10 <10 <10 <10

*Serum samples were obtained from mice 14 d after inoculation. HuN EA-H1N1, Eurasian avian-like influenza A/Hunan/42443/2015(H1N1) virus; JS/1/11 EA-H1N1, Eurasian avian-like influenza A/Jiangsu/1/2011(H1N1) virus; MID50, mouse infectious dose; ND, not determined due to death of the animal (treated as positive for determination of the MID50); TCID50, 50% tissue culture infectious dose.
†Titers <20 were regarded as negative for seroconversion.
‡MID50 was determined using the Spearman–Karber method (19).

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References
  1. Castrucci  MR, Donatelli  I, Sidoli  L, Barigazzi  G, Kawaoka  Y, Webster  RG. Genetic reassortment between avian and human influenza A viruses in Italian pigs. Virology. 1993;193:503–6.DOIPubMedGoogle Scholar
  2. Ma  W, Kahn  RE, Richt  JA. The pig as a mixing vessel for influenza viruses: Human and veterinary implications. J Mol Genet Med. 2008;3:158–66.PubMedGoogle Scholar
  3. Claas  EC, Kawaoka  Y, de Jong  JC, Masurel  N, Webster  RG. Infection of children with avian-human reassortant influenza virus from pigs in Europe. Virology. 1994;204:453–7.DOIPubMedGoogle Scholar
  4. Komadina  N, Roque  V, Thawatsupha  P, Rimando-Magalong  J, Waicharoen  S, Bomasang  E, Genetic analysis of two influenza A (H1) swine viruses isolated from humans in Thailand and the Philippines. Virus Genes. 2007;35:161–5.DOIPubMedGoogle Scholar
  5. Shinde  V, Bridges  CB, Uyeki  TM, Shu  B, Balish  A, Xu  X, Triple-reassortant swine influenza A (H1) in humans in the United States, 2005-2009. N Engl J Med. 2009;360:2616–25.DOIPubMedGoogle Scholar
  6. Zhu  WF, Shu  YL. [An overview of swine influenza virus infection in humans] [in Chinese]. Bing Du Xue Bao. 2013;29:559–65.PubMedGoogle Scholar
  7. Gaydos  JC, Hodder  RA, Top  FH Jr, Soden  VJ, Allen  RG, Bartley  JD, Swine influenza A at Fort Dix, New Jersey (January-February 1976). I. Case finding and clinical study of cases. J Infect Dis. 1977;136(Suppl):S356–62.DOIPubMedGoogle Scholar
  8. Hodder  RA, Gaydos  JC, Allen  RG, Top  FH Jr, Nowosiwsky  T, Russell  PK. Swine influenza A at Fort Dix, New Jersey (January-February 1976). III. Extent of spread and duration of the outbreak. J Infect Dis. 1977;136(Suppl):S369–75.DOIPubMedGoogle Scholar
  9. Qi  X, Cui  L, Jiao  Y, Pan  Y, Li  X, Zu  R, Antigenic and genetic characterization of a European avian-like H1N1 swine influenza virus from a boy in China in 2011. Arch Virol. 2013;158:39–53.DOIPubMedGoogle Scholar
  10. Zu  R, Dong  L, Qi  X, Wang  D, Zou  S, Bai  T, Virological and serological study of human infection with swine influenza A H1N1 virus in China. Virology. 2013;446:49–55.DOIPubMedGoogle Scholar
  11. Wang  DY, Qi  SX, Li  XY, Guo  JF, Tan  MJ, Han  GY, Human infection with Eurasian avian-like influenza A(H1N1) virus, China. Emerg Infect Dis. 2013;19:1709–11.DOIPubMedGoogle Scholar
  12. Yang  H, Chen  Y, Qiao  C, He  X, Zhou  H, Sun  Y, Prevalence, genetics, and transmissibility in ferrets of Eurasian avian-like H1N1 swine influenza viruses. Proc Natl Acad Sci U S A. 2016;113:392–7.DOIPubMedGoogle Scholar
  13. Chen  H, Yuan  H, Gao  R, Zhang  J, Wang  D, Xiong  Y, Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection: a descriptive study. Lancet. 2014;383:714–21.DOIPubMedGoogle Scholar
  14. Reed  LJ, Muench  H. A simple method of estimating fifty percent endpoints. Am J Hyg. 1938;27:493–7.
  15. Kendal  AP, Skehel  JJ, Pereira  MS. World Health Organization Collaborating Centers for Reference and Research on Influenza: concepts and procedures for laboratory-based influenza surveillance. Geneva: World Health Organization; 1982. p. B17–35.
  16. World Health Organization. WHO manual on animal influenza diagnosis and surveillance. WHO/ CDS/CSR/NCS/2002.5 [cited 2015 Dec 22]. http://apps.who.int/iris/bitstream/10665/68026/1/WHO_CDS_CSR_NCS_2002.5.pdf
  17. Nguyen  HT, Fry  AM, Gubareva  LV. Neuraminidase inhibitor resistance in influenza viruses and laboratory testing methods. Antivir Ther. 2012;17(1 Pt B):159–73.DOIPubMedGoogle Scholar
  18. Xie  Z, Zhang  M, Xie  L, Luo  S, Liu  J, Deng  X, Identification of a triple-reassortant H1N1 swine influenza virus in a southern China pig. Genome Announc. 2014;2:e00229–14.DOIPubMedGoogle Scholar
  19. Karber  G. 50% end point calculation. Archiv für Experimentelle Pathologie und Pharmakologie. 1931;162:480–3.
  20. Zhu  W, Yang  S, Guo  Y, Yang  L, Bai  T, Yu  Z, Imported pigs may have introduced the first classical swine influenza viruses into Mainland China. Infect Genet Evol. 2013;17:142–6.DOIPubMedGoogle Scholar
  21. Lakdawala  SS, Lamirande  EW, Suguitan  AL Jr, Wang  W, Santos  CP, Vogel  L, Eurasian-origin gene segments contribute to the transmissibility, aerosol release, and morphology of the 2009 pandemic H1N1 influenza virus. PLoS Pathog. 2011;7:e1002443.DOIPubMedGoogle Scholar

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1These authors contributed equally to this article.

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