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Volume 22, Number 2—February 2016
Research

Trematode Fluke Procerovum varium as Cause of Ocular Inflammation in Children, South India

Lalan Kumar Arya, Sivakumar R. Rathinam, Prajna Lalitha, Usha R. Kim, Sudeep Ghatani, and Veena Tandon1Comments to Author 
Author affiliations: Aravind Medical Research Foundation, Madurai, India (L.K. Arya, P. Lalitha); Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Madurai (S.R. Rathinam, U.R. Kim); North-Eastern Hill University, Shillong, India (S. Ghatani, V. Tandon)

Main Article

Figure 3

Real-time PCR amplification of ocular granuloma DNA obtained from patients infected with trematodes, South India. Gel electrophoresis was performed on 2% agarose gel by using Power SYBR Green Real-Time PCR (Applied Biosystems, Warrington, UK). A) Lanes 1–4 show subconjunctival granuloma DNA; lane 5, negative control; lane 6, 100-bp DNA marker. Arrow indicates 369-bp amplified DNA product. B) Lanes 1–5 show anterior chamber granuloma DNA; lane 6, negative control; lane 7, 100-bp DNA marker. Arrow

Figure 3. Real-time PCR amplification of ocular granuloma DNA obtained from patients infected with trematodes, South India. Gel electrophoresis was performed on 2% agarose gel by using Power SYBR Green Real-Time PCR (Applied Biosystems, Warrington, UK). A) Lanes 1–4 show subconjunctival granuloma DNA; lane 5, negative control; lane 6, 100-bp DNA marker. Arrow indicates 369-bp amplified DNA product. B) Lanes 1–5 show anterior chamber granuloma DNA; lane 6, negative control; lane 7, 100-bp DNA marker. Arrow indicates 369-bp amplified DNA product. C) BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi) analysis output of patient granuloma DNA sequence showing maximum identity with internal transcribed spacer 2 region gene sequence of the Procerovum species resembling GenBank reported sequence EU826639.1 from Vietnam.

Main Article

1Current affiliation: National Academy of Sciences, India, Lucknow, India.

Page created: January 13, 2016
Page updated: January 13, 2016
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