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Volume 7, Number 6—December 2001

Research

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†Comments to Author , Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*
Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

Main Article

Figure 1

Workflow for typing prn alleles. The allele-specific amplification (ASA) assay (step number 3) and the fluorescence resonance energy transfer (FRET) probe assay (step number 4) each require approximately 1 hour.

Figure 1. Workflow for typing prn alleles. The allele-specific amplification (ASA) assay (step number 3) and the fluorescence resonance energy transfer (FRET) probe assay (step number 4) each require approximately 1 hour.

Main Article

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