Emerging Infectious Disease ISSN: 1080-6059

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†

, Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*

Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

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Figure 4

A. Nucleotide sequences of polymorphic regions of different types of the prn gene and the sequences of the fluorescence resonance energy transfer probes aligned to their hybridization positions in the prn gene. Number 790 refers to the position of bases relative to the first start codon of prn1. Underlined regions represent repeats in the sequence. B. Amino acid sequences of polymorphic regions of different pertactin types. Dashes indicate caps in the sequence.

Figure 4. A. Nucleotide sequences of polymorphic regions of different types of the prn gene and the sequences of the fluorescence resonance energy transfer probes aligned to their hybridization positions in the prn gene. Number 790 refers to the position of bases relative to the first start codon of prn1. Underlined regions represent repeats in the sequence. B. Amino acid sequences of polymorphic regions of different pertactin types. Dashes indicate caps in the sequence.

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