Emerging Infectious Disease ISSN: 1080-6059

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†

, Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*

Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

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Figure 5

A. Melting curves from the allele-specific amplification assay, showing the presence of amplified products from prn1-5 and the absence of amplification from prn6-8 and the negative control. B. Corresponding melting peaks derived from the melting curve. Prn1 represents the prn1-5 types; prn7 represents prn6-8 types and the negative control.

Figure 5. A. Melting curves from the allele-specific amplification assay, showing the presence of amplified products from prn1-5 and the absence of amplification from prn6-8 and the negative control. B. Corresponding melting peaks derived from the melting curve. Prn1 represents the prn1-5 types; prn7 represents prn6-8 types and the negative control.

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