Emerging Infectious Disease ISSN: 1080-6059

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†

, Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*

Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

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Figure 6

Curves showing the dissociation of fluorescence resonance energy transfer probe assay probes from the polymerase chain reaction products of different prn types. Negative control includes all reagents but no template DNA.

Figure 6. Curves showing the dissociation of fluorescence resonance energy transfer probe assay probes from the polymerase chain reaction products of different prn types. Negative control includes all reagents but no template DNA.

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