Emerging Infectious Disease ISSN: 1080-6059

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†

, Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*

Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

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Figure 7

Ethidium bromide stained 3% molecular screening agarose gel containing Bordetella pertussis DNA amplified with primers QH8F´ and QH2R. Lanes: 1, negative control including all reagents but no template DNA; 2, 100-bp ladder; 3, B. pertussis strain 1772 of type prn1 (260 bp); 4, Bordetella pertussis clinical isolate of type prn2 (275 bp); 5, B. pertussis clinical isolate of type prn3 (260 bp); 6, B. pertussis clinical isolate of type prn4 (245 bp); and 7, B. pertussis type prn5 (245 bp).

Figure 7. Ethidium bromide stained 3% molecular screening agarose gel containing Bordetella pertussis DNA amplified with primers QH8F´ and QH2R. Lanes: 1, negative control including all reagents but no template DNA; 2, 100-bp ladder; 3, B. pertussis strain 1772 of type prn1 (260 bp); 4, Bordetella pertussis clinical isolate of type prn2 (275 bp); 5, B. pertussis clinical isolate of type prn3 (260 bp); 6, B. pertussis clinical isolate of type prn4 (245 bp); and 7, B. pertussis type prn5 (245 bp).

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