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Volume 7, Number 6—December 2001

Research

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†Comments to Author , Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*
Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

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Table 2

Comparison of the results from sequencing and the hybridization probe assay in the determination of the pertactin gene type of Bordetella pertussis strains


Melting temperature
Melting curve
Pertactin allele typea (determined by sequencing) No. of isolates Mean SD Area SD
1 9 58.78b 0.26 21.64b 5.64
2 25 56.31 0.44 1.25 0.30
3 4 56.59 0.09 1.35 0.26
4 5 56.57 0.29 1.43 0.25
5 1 - - - -

aOf the strains representing prn1-4, 41 were Finnish clinical isolates, and 2 were Finnish vaccine strains.
bAll P values were <0.0001 when prn1 was compared to prn2, prn3, or prn4.

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