Volume 8, Number 3—March 2002
Research
Eastern Equine Encephalomyelitis Virus Infection in a Horse from California
Robert P. Franklin*
, Hailu Kinde†, Michele T. Jay‡, Laura D. Kramer†, Emily-Gene N. Green†, Robert E. Chiles†, Eileen Ostlund§, Stan Husted‡, Jonathan Smith¶, and Michael D. Parker¶
, Hailu Kinde†, Michele T. Jay‡, Laura D. Kramer†, Emily-Gene N. Green†, Robert E. Chiles†, Eileen Ostlund§, Stan Husted‡, Jonathan Smith¶, and Michael D. Parker¶Table 1
Reverse transcriptase-polymerase chain reaction (RT-PCR) primers used to sequence Eastern equine encephalomyelitis virus RNA
| Title | Sense | Primer | Use | Region |
|---|---|---|---|---|
| 9930 | Forward | 5´- GCGCTGCTTATTTTGCTGT -3 | RT-PCR | E1 |
| 11582 | Reverse | 5´- ATTATGCGCTGCCTGTAGTGTTTA –3´ | RT-PCR | E1 |
| 4043 | Forward | 5´- GTGCGCGGCGACATAACAAAGAG- 3´ | RT-PCR | NSP3 |
| 4976 | Reverse | 5´- CCGGGGGTACAGTGCCAGAGAA –3´ | RT-PCR | NSP3 |
| 10470 | Forward | 5´- ATGCCAAACTCATCATAGGTCCACT –3´ | Sequencing | E1 |
| 10938 | Forward | 5´- GTATAGCCACCGTTGCCTACAAATC –3´ | Sequencing | E1 |
| 11345 | Forward | 5´- CAGGCAGTGTATAAGGCTGTCTTAC –3´ | Sequencing | E1 |
| T3 | Forward | 5´- AATTAACCCTCACTAAAGGGA –3´ | Sequencing | NSP3 |
