Volume 8, Number 3—March 2002
Research
Immunization with Heterologous Flaviviruses Protective Against Fatal West Nile Encephalitis
Robert B. Tesh
, Amelia P.A. Travassos da Rosa, Hilda Guzman, Tais P. Araujo, and Shu-Yuan Xiao
, Amelia P.A. Travassos da Rosa, Hilda Guzman, Tais P. Araujo, and Shu-Yuan XiaoTable 2
Serologic response of adult hamsters to West Nile virus (WNV), Yellow fever virus (YFV), St. Louis encephalitis virus (SLEV), and Japanese encephalitis virus (JEV) antigens, at various intervals after intraperitoneal inoculation of 104.0 TCID50 of WNV
| HI antibody titer | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Animal no. | WNV | YFV | SLEV | JEV | WN MAC-ELISA | ||||||
| Day 6 | |||||||||||
| 8251(Da) | 1:40 | 0b | 0 | 0 | 0.633c | ||||||
| 8252 | 1:80 | 0 | 0 | 0 | 1.013 | ||||||
| 8253(D) | 1:160 | 0 | 0 | 0 | 0.878 | ||||||
| 8254 | 1:160 | 0 | 0 | 0 | 1.090 | ||||||
| 8255 | 1:80 | 0 | 0 | 0 | 0.848 | ||||||
| 8256 | 1:80 | 0 | 0 | 0 | 0.840 | ||||||
| 8257(D) | 1:160 | 0 | 0 | 0 | 1.291 | ||||||
| 8258 | 1:80 | 0 | 0 | 0 | 0.869 | ||||||
| 8259(D) | 1:160 | 0 | 0 | 0 | 0.939 | ||||||
| 8260 | 1:80 | 0 | 0 | 0 | 0.992 | ||||||
| 8262 | 1:80 | 0 | 0 | 0 | 0.748 | ||||||
| 8263 | 1:80 | 0 | 0 | 0 | 0.797 | ||||||
| 8264(D) | 1:80 | 0 | 0 | 0 | 0.827 | ||||||
| Day 31 | |||||||||||
| 8252 | 1:1,280 | 1:320 | 1:320 | 1:320 | 0.401 | ||||||
| 8254 | 1:1,280 | 1:320 | 1:320 | 1:320 | 0.427 | ||||||
| 8255 | 1:640 | 1:160 | 1:320 | 1:160 | 0.488 | ||||||
| 8256 | 1:640 | 1:160 | 1:160 | 1:160 | 0.582 | ||||||
| 8258 | 1:640 | 1:160 | 1:160 | 1:160 | 0.376 | ||||||
| 8260 | 1:1,280 | 1:320 | 1:320 | 1:320 | 0.420 | ||||||
| 8262 | 1:1,280 | 1:320 | 1:320 | 1:320 | 0.246 | ||||||
| 8263 | 1:640 | 1:160 | 1:160 | 1:160 | 0.516 | ||||||
| Day 60 | |||||||||||
| 8252 | 1:2,560 | 1:640 | 1:640 | 1:640 | 0.269 | ||||||
| 8255 | 1:2,560 | 1:640 | 1:640 | 1:640 | 0.216 | ||||||
| 8256 | 1:640 | 1:160 | 1:160 | 1:160 | 0.162 | ||||||
| 8258 | 1:1,280 | 1:320 | 1:320 | 1:320 | 0.161 | ||||||
| 8260 | 1:320 | 1:80 | 1:80 | 1:40 | 0.179 | ||||||
| 8262 | 1:640 | 1:160 | 1:160 | 1:160 | 0.181 | ||||||
| Day 90 | |||||||||||
| 8254 | 1:320 | 1:80 | 1:80 | 1:80 | 0.167 | ||||||
| 8260 | 1:640 | 1:80 | 1:80 | 1:80 | 0.217 | ||||||
| 8262 | 1:640 | 1:80 | 1:160 | 1:160 | 0.184 | ||||||
a(D): Hamster died of encephalitis 7 to 14 days after infection. HI = hemagglutination inhibition.
b0 <1:20.
cOptical density value (>0.200 is positive).
