Figure 1. The open view of diagnostic electron microscopy. A. Multiple agents observed in a fecal sample from a pediatric patient with diarrhea. A 10% suspension was prepared in distilled water, cleared by low-speed centrifugation followed by 5 minutes at 15,000 x g in a bench top centrifuge, and centrifuged directly to the grid using an Airfuge EM-90 rotor (Beckman, Palo Alto, CA): adenovirus-(→), incomplete rotavirus-particle (>), and small round featureless particles, probably adeno-associated virus (ρ) phosphotungstic acid stained. B. Double infection with adenovirus (→) and complete rotavirus particles (>), in the stool of a 1-yearold child. The sample was suspended 1:3 in distilled water, cleared by low-speed centrifugation, and prepared for examination by the two-step method. Aqueous uranyl acetate stained. Bar = 100 nm.