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Volume 9, Number 5—May 2003

Research

Entamoeba moshkovskii Infections in Children in Bangladesh

Ibne Karin M. Ali*†1, Mohammad Bakhtiar Hossain†1, Shantanu Roy†, Patrick F. Ayeh-Kumi‡, William A. Petri§, Rashidul Haque†, and C. Graham Clark*Comments to Author 
Author affiliations: *London School of Hygiene and Tropical Medicine, London, England; †International Centre for Diarrheal Disease Research, Dhaka, Bangladesh; ‡University of Ghana Medical School, Accra, Ghana; §University of Virginia School of Medicine, Charlottesville, Virginia, USA; 1These authors contributed equally to this work.

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Table 1

Oligonucleotide primers used to detect Entamoeba histolytica and E. dispar in stool specimens

Primer Primer sequence (5´ to 3´)
E-1 TTT GTA TTA GTA CAA A
E-2 GTA [A/G]TA TTG ATA TAC T
Eh-1 AAT GGC CAA TTC ATT CAA TG
Eh-2 TTT AGA AAC AAT GCT TCT CT
Ed-1 AGT GGC CAA TTT ATG TAA GT
Ed-2 TTT AGA AAC AAT GTT TCT TC
Em-1 CTC TTC ACG GGG AGT GCG
Em-2 TCG TTA GTT TCA TTA CCT
nEm-1 GAA TAA GGA TGG TAT GAC
nEm-2 AAG TGG AGT TAA CCA CCT
ArgTCT-1 AGC ATC AGC CTT CTA AGC TG
ArgTCT-2 CTT CCG ACT GAG CTA ACA AG
EmR-1 GGC GCC TTT TTT ACT TTA TGG
EmR-2 GCT AAC AAG GCC AAT CGA TAA A

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