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Volume 9, Number 5—May 2003

Research

Entamoeba moshkovskii Infections in Children in Bangladesh

Ibne Karin M. Ali*†1, Mohammad Bakhtiar Hossain†1, Shantanu Roy†, Patrick F. Ayeh-Kumi‡, William A. Petri§, Rashidul Haque†, and C. Graham Clark*Comments to Author 
Author affiliations: *London School of Hygiene and Tropical Medicine, London, England; †International Centre for Diarrheal Disease Research, Dhaka, Bangladesh; ‡University of Ghana Medical School, Accra, Ghana; §University of Virginia School of Medicine, Charlottesville, Virginia, USA; 1These authors contributed equally to this work.

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Table 2

Nested SSU rDNA polymerase chain reaction (PCR) (for Entamoeba histolytica, E. dispar, or both) and stool antigen-detection test results of the 17 E. moshkovskii–positive samplesa

Samples Stool antigen-detection test results: SSU rRNA gene PCR for E. histolytica/E. dispar
Stool DNA Culture DNA
1b
E. histolytica
E. dispar
Mixed
2
E. dispar
0
NC
3
E. dispar
E. dispar
NC
4
E. histolytica
0
NC
5
E. dispar
E. dispar
E. dispar
6c
E. dispar
Mixed
E. dispar
7
E. dispar
E. dispar
NC
8
E. dispar
E. dispar
NC
9
E. dispar
E. dispar
NC
10
0
E. dispar
NC
11
E. dispar
E. dispar
NC
12
E. dispar
0
NC
13c
E. dispar
E. histolytica
NC
14
0
0
E. dispar
15
E. dispar
0
E. dispar
16
0
0
E. dispar
17
E. histolytica
0
NC
18
0
0
NC
19
0
0
NC
20
0
0
NC
21
0
0
NC
22
0
0
NC
23 0 0 NC

aNC, no culture; 0, negative. All stool antigen tests that are positive for E. histolytica can also be mixed because no specific E. dispar antigen test exists.
bPatients 1 and 6 likely had mixed infections with E. histolytica and E. dispar, in which E. histolytica was much lower in number than E. dispar in the stool specimen. For patient 1, SSU rDNA PCR failed to detect E. histolytica, though both species grew in the culture. For patient 6, although SSU rDNA PCR could detect E. histolytica in stool DNA, the E. histolytica antigen-detection test failed to detect E. histolytica, and only E. dispar survived in the culture.
cThe stool specimen of patient 13 was marginally negative by the E. histolytica antigen-detection test (optical density value was 0.13 where the cut-off value for a positive result was 0.15).

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