Volume 11, Number 1—January 2005
Research
Mycobacterium haemophilum and Lymphadenitis in Children
Lesla E.S. Bruijnesteijn van Coppenraet*, Edward J. Kuijper*
, Jerome A. Lindeboom†, Jan M. Prins†, and Eric C. J. Claas*
, Jerome A. Lindeboom†, Jan M. Prins†, and Eric C. J. Claas*Table 1
Sequences of oligonucleotides used in this study
| Primer/probe sequence (5′–3′) target sequence | ||
|---|---|---|
| ITS forward primer real-time PCR | GGGGTGTGGTGTTTGAG | Partial ITS |
| ITS reverse primer real-time PCR | CTCCCACGTCCTTCATC | Partial ITS |
| Forward primer Ec16S.1390p* | TTGTACACACCGCCCGTCA | Total ITS |
| Reverse primer Mb23S.44n* | TCTCGATGCCAAGGCATCCACC | Total ITS |
| 16S forward primer P1† | TAACACATGCAAGTCGAACG | 16S |
| 16S reverse primer P4† | TCGTTGCGGGACTTAACCCAAC | 16S |
| Mycobacterium genus–specific TaqMan probe | Fam-GGATAGTGGTTGCGAGCATC-Tamra | ITS |
| M. haemophilum–specific MGB-probe | VIC–ACGCCACCATTACT-MGB | ITS |
*Primers published in (19).
†Primers published in (23).
