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Volume 11, Number 10—October 2005


Detecting Biological Warfare Agents

Linan Song*, Soohyoun Ahn*, and David R. Walt*Comments to Author 
Author affiliations: *Tufts University, Medford, Massachusetts, USA

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Table A3

Number of microorganisms in autoclaved bacterial cultures used as templates in polymerase chain reaction; cultures were also used to spike wastewater and prepare mixed bacterial samples*

Organism DNA copies/mL
Bacillus thuringiensis kurstaki 2.4 × 108
B. anthracis Ames 1.2 × 105
Vaccinia virus 7.7 × 106
Yersinia pestis CO-92 2.8 × 105
Clostridium botulinum A 3.1 × 107
Francisella tularensis Novicida 1.1 × 106

*Naval Medical Research Command (NMRC) did not provide an autoclaved bacterial culture of Brucella melitensis; therefore, no results were obtained for Brucella melitensis other than with synthetic target sequences. The numbers provided by NMRC in this table are based on real- time polymerase chain reaction quantification, where a standard curve was produced with cloned material and used to calculate the results of a gene copy/mL quantification of the autoclaved material.

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