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Volume 12, Number 6—June 2006


West Nile Virus Infection in Humans and Horses, Cuba

Maritza Pupo*Comments to Author , Maria Guadalupe Guzmán*, Roberto Fernández*, Alina Llop*, Félix Orlando Dickinson*, Daniel Pérez*, Raúl Cruz†, Tayri González†, Gonzalo Estévez†, Hiram González‡, Paulino Santos§, Gustavo Kourí*, Maya Andonova¶, Robbin Lindsay¶, Harvey Artsob¶, and Michael Drebot¶
Author affiliations: *Tropical Medicine Institute "Pedro Kouri," Havana, Cuba; †Ministry of Public Health, Havana, Cuba; ‡Instituto de Ecología y Sistemática, Havana, Cuba; §Instituto de Medicina Veterinaria, Havana, Cuba; ¶National Microbiology Laboratory, Winnipeg, Manitoba, Canada

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Table 2

Summary of human sera tested for evidence of WNV infection by ELISA and PRNT*

Patient code ELISA result†
90% PRNT titers
IgM IgG HI titer† WNV SLEV DENV Interpretation
M.M. +/+ +/+ 40/160 320 WNV
K.R. +/+ +/+ 20/80 160 WNV
O.G. -/- +/+ 20/20 40 WNV
M.L. -/- +/+ 20/20 640 80 SLEV
A.M.L. -/- +/+ 20/40 320 SLEV
R.A. ND/– ND/+ 1,280 80 SLEV
P.Y. ND/– ND/+ <20 40 SLEV
M.V. -/- +/+ 20/40 80 SLEV
J.A.R. -/- +/+ 20/40 40 80 80 Unidentified flavivirus
R.C. -/- +/+ 20/20 80 DENV
A.P. -/- +/+ 20/20 Unidentified flavivirus
L.V. -/- +/+ 20/40 Unidentified flavivirus
D.B. ND/– ND/+ ND Unidentified flavivirus

*WNV, West Nile virus; ELISA, enzyme-linked immunosorbent assay; PRNT, plaque reduction neutralization test; Ig, immunoglobulin; HI, hemagglutination inhibition; SLEV, Saint Louis encephalitis virus; DENV, dengue virus; ND, not done.
†Acute-phase/convalescent-phase serum samples.

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