Volume 14, Number 8—August 2008
Research
Puumala Hantavirus Excretion Kinetics in Bank Voles (Myodes glareolus)
Jonas Hardestam*†1
, Malin Karlsson*1, Kerstin I. Falk*†, Gert Olsson*‡§, Jonas Klingström*†, and Åke Lundkvist*†
, Malin Karlsson*1, Kerstin I. Falk*†, Gert Olsson*‡§, Jonas Klingström*†, and Åke Lundkvist*†Table 1
Detection of PUUV RNA over time in excreta from bank voles subcutaneously inoculated with PUUV strain Kazan wild type*
| Bank vole no. | Excretion type | Detection of PUUV RNA in excreta by time, d | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 8 | 9 | 11 | 14 | 16 | 21 | 28 | 35 | 44 | 49 | 56 | 63 | 70 | 77 | 84 | 91 | 133 | ||
| 1 | Saliva | – | – | – | – | + | – | – | – | – | + | + | – | – | – | – | – | – | – | – | – | – |
| Urine | NT | – | NT | NT | NT | NT | – | – | – | NT | + | – | NT | NT | – | NT | NT | – | NT | NT | – | |
| Feces | – | – | – | – | – | – | + | – | – | – | – | + | – | – | – | – | – | – | – | – | – | |
| 2 | Saliva | – | – | – | – | – | + | + | + | + | + | + | – | – | + | – | – | – | + | – | – | – |
| Urine | – | NT | – | NT | NT | NT | – | NT | NT | – | – | – | NT | NT | NT | NT | – | NT | – | NT | NT | |
| Feces | – | – | – | – | – | – | – | – | – | + | + | – | + | – | – | – | – | – | – | – | – | |
| 3 | Saliva | – | – | – | – | – | + | + | + | + | + | + | + | + | + | + | + | – | – | + | – | – |
| Urine | – | – | NT | NT | – | NT | + | NT | + | NT | – | – | + | NT | NT | NT | – | NT | NT | NT | NT | |
| Feces | – | – | – | – | – | – | – | – | + | + | + | – | – | – | – | – | – | – | – | – | – | |
| 4† | Saliva | – | – | – | – | – | – | – | + | + | + | |||||||||||
| Urine | – | – | NT | NT | NT | NT | – | – | – | + | ||||||||||||
| Feces | – | – | – | – | – | – | – | + | + | + | ||||||||||||
| 5‡ | Saliva | – | – | – | – | – | – | + | + | + | + | + | + | + | – | + | – | – | – | – | – | |
| Urine | NT | – | NT | – | NT | NT | + | + | NT | NT | NT | NT | NT | – | – | NT | – | NT | NT | NT | ||
| Feces | – | – | – | – | – | – | – | – | + | – | + | – | – | – | – | – | – | – | – | – | ||
| 6 | Saliva | – | – | – | – | – | – | + | + | + | + | + | + | + | + | + | – | – | - | + | – | – |
| Urine | – | NT | – | NT | NT | – | NT | NT | + | NT | NT | + | – | NT | – | NT | – | NT | - | – | NT | |
| Feces | – | – | – | – | – | – | – | – | + | + | – | – | + | – | – | – | – | – | – | – | – | |
| 7 | Saliva | – | – | – | – | – | – | + | + | + | + | + | + | + | + | – | – | – | – | – | – | – |
| Urine | – | NT | NT | NT | NT | – | – | + | + | + | – | NT | – | NT | – | NT | – | NT | – | NT | NT | |
| Feces | – | – | – | – | – | – | – | + | + | + | – | - | – | – | – | – | – | – | – | – | – | |
| 8 | Saliva | – | – | – | – | – | – | + | + | + | + | + | + | + | + | – | – | – | – | – | – | – |
| Urine | – | – | – | NT | NT | – | – | – | + | – | NT | – | – | – | – | – | NT | – | – | NT | – | |
| Feces | – | – | – | – | – | – | – | + | – | – | – | – | – | – | – | – | – | – | – | –- | – | |
| 9† | Saliva | – | – | – | – | – | – | – | – | + | + | |||||||||||
| Urine | NT | NT | – | – | – | – | – | NT | + | + | ||||||||||||
| Feces | – | – | – | – | – | – | – | – | –- | + | ||||||||||||
| 10§ | Saliva | – | – | – | – | – | + | + | + | + | + | + | + | |||||||||
| Urine | NT | NT | – | NT | NT | NT | NT | – | + | + | – | + | ||||||||||
| Feces | – | – | – | – | – | – | – | – | – | – | – | – | ||||||||||
*PUUV, Puumala virus; –, negative; +, positive, NT, not tested (no or insufficient urine volume).
†Died on day 21 postinfection.
‡Died on day 112 postinfection.
§Died on day 35 postinfection.
