Volume 15, Number 12—December 2009
Dispatch
Diagnostic Assay for Rickettsia japonica
Nozomu Hanaoka, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, and Shuji Ando
Table 1
Reactivity of the real-time PCR for Rickettsia strains used in this study*
| Species | Strain | Isolation source | Reference | Real-time PCR |
|---|---|---|---|---|
| Rickettsia asiatica | IO-1 | Ixodes ovatus | ATCC VR-1593 (8) | – |
| IO-2 | I. ovatus | (8) | – | |
| IO-3 | I. ovatus | (8) | – | |
| IO-25 | I. ovatus | (8) | – | |
| IO-38 |
I. ovatus |
(8) |
– |
|
| R. conorii |
Malish 7 |
Human |
ATCC VR 613T |
– |
| R. heilongjiangensis |
CH8-1 |
Haemaphysalis concinna |
(6) |
+ |
| R. helvetica | IM-1 | Ixodes monospinosus | (9) | – |
| IP-1 | I. persulcatus | (9) | – | |
| IP-2 | I. persulcatus | (10) | – | |
| IP-6 |
I. persulcatus |
This study |
– |
|
| R. honei |
TT-118 |
Ixodes sp. |
(11) |
– |
| R. japonica | DT-1 | Dermacentor taiwanensis | (12) | + |
| YH | Human | ATCC VR-1363 | + | |
| FLA-1 | Haemaphysalis flava | (9) | + | |
| HH-8 | H. hystricis | This study | + | |
| HH-9 |
H. hystricis |
This study |
+ |
|
| R. prowazekii |
breinl |
Human |
(13) |
– |
| R. rickettsii |
Sheila Smith |
Human |
(14) |
– |
| R. sibirica |
246 |
Human |
ATCC VR-151 |
– |
| R. tamurae | AT-1 | Amblyomma testudinarium | ATCC VR-1594 (12) | – |
| AT-4 | A. testudinarium | (6) | – | |
| AT-13 |
A. testudinarium |
(6) |
– |
|
| R. typhi |
Wilmington |
Human |
ATCC VR-144 |
– |
| Rickettsia sp. LON | LON-2 | Haemaphysalis longicornis | (6) | – |
| LON-9 | H. longicornis | (6) | – | |
| LON-13 | H. longicornis | (6) | – |
*+, positive; –, not detected.
