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Volume 15, Number 9—September 2009

Research

Susceptibilities of Nonhuman Primates to Chronic Wasting Disease

Brent Race1Comments to Author , Kimberly D. Meade-White1, Michael W. Miller, Kent D. Barbian, Richard Rubenstein, Giuseppe LaFauci, Larisa Cervenakova, Cynthia Favara, Donald Gardner, Dan Long, Michael Parnell, James Striebel, Suzette A. Priola, Anne Ward, Elizabeth S. Williams2, Richard Race3, and Bruce Chesebro3
Author affiliations: Rocky Mountain Laboratories, Hamilton, Montana, USA (B. Race, K.D. Meade-White, K.D. Barbian, C. Favara, D. Gardner, D. Long, M. Parnell, J. Striebel, S.A. Priola, A. Ward, R. Race, B. Chesebro); Colorado Division of Wildlife, Fort Collins, Colorado, USA (M.W. Miller); State University of New York Downstate Medical Center, Brooklyn, New York, USA (R. Rubenstein); New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York, USA (G. LaFauci); American Red Cross, Rockville, Maryland, USA (L. Cervenakova); University of Wyoming, Laramie, Wyoming, USA (E.S. Williams)

Main Article

Table 1

Results of squirrel monkey intracerebral inoculation with CWD agent*

Monkey no.† PrP genotype‡ CWD inoculum Titer inoculated§ Incubation period, mpi¶ Weight change, %
308 NT MD-1 1.0 × 106 36 –8
633 A MD-1 1.0 × 107 36 –42
334 B MD-2 6.4 × 105 43 –38
393 B MD-2 6.4 × 105 46 –28
640 A MD-3 2.0 × 106 44 –35
365 NT Elk-1 1.3 × 105 40 –43
643 A Elk-1 1.3 × 106 53 –27
321 NT Elk-2 4.0 × 105 35 –23
322 NT Elk-3 2.6 × 105 33 –40
624 A Elk-3 2.6 × 106 48 –37
399 A WTD-1 8.0 × 106 50 –33
628 NT WTD-1 8.0 × 106 NS (52) 0
310 A WTD-2 1.3 × 105 NS (69) +7
319 A Normal elk NS (69) –8

*CWD, chronic wasting disease; PrP, prion protein; mpi, months postinfection; NT, not tested (sequenced); NS, no signs.
†In addition to the monkeys listed, 4 asymptomatic squirrel monkeys were euthanized at 10 mo after intracerebral inoculation with MD-1, MD-3, Elk-1, and WTD-1 to detect early accumulation of protease-resistant PrP (PrPres), but no PrPres was detected in brain by Western blot.
‡See Table 4 for a description of genotypes A, B, and C.
§Infectivity titers were determined by using endpoint dilution titer in transgenic deer expressing mouse PrP and are the 50% infectious dose/g of brain.
¶Incubation periods for monkeys with clinical wasting are indicated as mpi in parentheses. NS indicates that these monkeys did not show any clinical signs compatible with transmissible spongiform encephalopathy or wasting.

Main Article

1These authors contributed equally to this article.

2Deceased.

3Co-senior authors.

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