Volume 15, Number 9—September 2009
Susceptibilities of Nonhuman Primates to Chronic Wasting Disease
|Monkey no.†||PrP genotype‡||CWD inoculum||Titer inoculated§||Incubation period, mpi¶||Weight change, %|
|308||NT||MD-1||1.0 × 106||36||–8|
|633||A||MD-1||1.0 × 107||36||–42|
|334||B||MD-2||6.4 × 105||43||–38|
|393||B||MD-2||6.4 × 105||46||–28|
|640||A||MD-3||2.0 × 106||44||–35|
|365||NT||Elk-1||1.3 × 105||40||–43|
|643||A||Elk-1||1.3 × 106||53||–27|
|321||NT||Elk-2||4.0 × 105||35||–23|
|322||NT||Elk-3||2.6 × 105||33||–40|
|624||A||Elk-3||2.6 × 106||48||–37|
|399||A||WTD-1||8.0 × 106||50||–33|
|628||NT||WTD-1||8.0 × 106||NS (52)||0|
|310||A||WTD-2||1.3 × 105||NS (69)||+7|
|319||A||Normal elk||NS (69)||–8|
*CWD, chronic wasting disease; PrP, prion protein; mpi, months postinfection; NT, not tested (sequenced); NS, no signs.
†In addition to the monkeys listed, 4 asymptomatic squirrel monkeys were euthanized at 10 mo after intracerebral inoculation with MD-1, MD-3, Elk-1, and WTD-1 to detect early accumulation of protease-resistant PrP (PrPres), but no PrPres was detected in brain by Western blot.
‡See Table 4 for a description of genotypes A, B, and C.
§Infectivity titers were determined by using endpoint dilution titer in transgenic deer expressing mouse PrP and are the 50% infectious dose/g of brain.
¶Incubation periods for monkeys with clinical wasting are indicated as mpi in parentheses. NS indicates that these monkeys did not show any clinical signs compatible with transmissible spongiform encephalopathy or wasting.