Volume 15, Number 9—September 2009
Susceptibilities of Nonhuman Primates to Chronic Wasting Disease
|Donor†||Original inoculum||Donor PrPres‡||TSE disease incidence§
|tg33 (deer)||tg66 (human)||tgRM (human)|
|Elk-3||NA||+||6/6 (301 ± 11)||NT||NT|
|MD-1||NA||+||7/7 (323 ± 15)||NT||NT|
|sCJD (97–008)||NA||+||NT||NT||4/6 (170 ± 3)|
|sCJD (99–009)||NA||+||NT||NT||5/5 (194 ± 20)|
|sCJD (RR)||NA||+||NT||8/8 (163 ± 1)||NT|
|sCJD (PLG)||NA||+||NT||4/4 (163 ± 6)||NT|
*CWD, chronic wasting disease; PrP, prion protein; TSE, transmissible spongiform encephalopathy; NT, not tested; sCJD, sporadic Creutzfeldt-Jakob disease.
†Each donor monkey inoculum was prepared as a 1% brain homogenate from the indicated monkeys. SM, squirrel monkey; CM, cynomolgus macaque. Elk and mule deer CWD inocula were described in Materials and Methods. Human sCJD inocula are brain homogenates from World Health Organization CJD reference materials. No. 99–009 is sCJD M/M type I, and no. 97–008 is sCJD M/M type II. The RR sample was from a patient with sCJD of unknown PrP genotype. The PLG sample (M/M type I) was from a patient with sCJD. In all cases except sCJD (RR), 50 μL was inoculated intracerebrally into recipient mice; for sCJD (RR), 30 μL was inoculated.
‡Based on Western blot or immunohistochemical analysis of brain for all except monkey 301, in which protease-resistant PrP (PrPres) was detected in spleen but not brain.
§Number of recipient mice with clinical transmissible spongiform encephalopathy confirmed by detection of brain PrPres is the numerator, and total number of mice inoculated is the denominator. Mean ± SEM incubation period for time to clinical disease is provided in days. Tg33 mice express deer PrP, and tg66 and tgRM mice express human PrP.
¶In addition to brain homogenate, we also inoculated tg33 mice with homogenates of spleen, lymph nodes, heart, muscle, and plasma from squirrel monkeys 322, 308, and 321, but disease did not develop during >600 d observation.