Volume 16, Number 4—April 2010
Use of Norovirus Genotype Profiles to Differentiate Origins of Foodborne Outbreaks
Because secondary transmission masks the connection between sources and outbreaks, estimating the proportion of foodborne norovirus infections is difficult. We studied whether norovirus genotype frequency distributions (genotype profiles) can enhance detection of the sources of foodborne outbreaks. Control measures differ substantially; therefore, differentiating this transmission mode from person-borne or food handler–borne outbreaks is of public health interest. Comparison of bivalve mollusks collected during monitoring (n = 295) and outbreak surveillance strains (n = 2,858) showed 2 distinguishable genotype profiles in 1) human feces and 2) source-contaminated food and bivalve mollusks; genotypes I.2 and I.4 were more frequently detected in foodborne outbreaks. Overall, ≈21% of all outbreaks were foodborne; further analysis showed that 25% of the outbreaks reported as food handler–associated were probably caused by source contamination of the food.
Noroviruses are members of the family Caliciviridae and recognized as major pathogens in outbreaks of gastroenteritis worldwide. Because these viruses have environmental stability (1), ability to use different transmission routes, and low infective doses (2), their source may be difficult to determine during an outbreak. Transmission can occur through contact with shedding persons; food contaminated during processing, preparation or serving; sewage-contaminated water used for consumption, cultivation or irrigation of food; contaminated aerosols resulting from vomiting; and environmental contamination (3,4). Five genogroups have been described (GI–V), subdivided into at least 40 genetic clusters (5,6).
To implement effective measures for prevention, recognition of the transmission routes is necessary. Consequently, the relative importance of different transmission routes in the total number of outbreaks is of interest for estimation of cost-effectiveness of reducing the number and size of norovirus outbreaks, particularly for geographically disseminated foodborne outbreaks. Such outbreaks are difficult to detect when the primary introduction of viruses through food occurs simultaneously in several countries or continents (7–9). Globalization of the food industry with consequential international distribution of products increases the risk for such outbreaks. For example, the first reported GII.b outbreak occurred in August 2000 during a large waterborne outbreak in southern France (10). After this outbreak, in December and January, 4 multipathogen and oyster-related outbreaks with this newly emerging genotype were reported from France. In the same period, Denmark, Finland, and the Netherlands reported norovirus cases resulting from oysters originating from a French batch that probably was sold in these countries, as well as in Sweden, Italy, and Belgium (6). All these outbreaks seemed to involve closely related and newly detected GII.b strains. After active case identification, further linked cases were detected in Germany, the United Kingdom, Spain, Slovenia, and Sweden (11,12). Another example of a geographically disseminated outbreak was several seemingly independent norovirus outbreaks in Denmark that were traced back to consumption of raspberries from Poland. Although raspberries from this contaminated batch were exported to other European countries, an alert in the Rapid Alert System for Food and Feed did not result in further linked outbreak reports (7). Thus, geographically disseminated outbreaks are sometimes identified but only after the joint and exhaustive efforts of different organizations, such as laboratory networks, food safety authorities, and public health institutions. Knowledge of the proportion of geographically disseminated foodborne outbreaks to all norovirus outbreaks will therefore provide insight into the cost-effectiveness of such efforts.
We studied whether the genotype frequency distributions (genotype profiles) of strains can be used to differentiate foodborne outbreaks related to contamination early in the food chain (i.e., during primary production) from those related to contamination later in the food chain (i.e., during preparation or serving). If so, detection of food origins likely to cause geographically disseminated outbreaks will be enhanced. We considered methods for attribution to multiple sources commonly applied to Salmonella infections (13) because different transmission routes involved in norovirus infections can disguise the foodborne origin. However, such methods require strain collections representative of noroviruses in the potential sources that are as yet unavailable because of difficulties in the direct detection of viruses in food (14–16). Therefore, we compared 2 strain collections: noroviruses identified through filter-feeding bivalve mollusk monitoring representing source contamination of food and noroviruses collected through systematic surveillance of illness in the population. The first was collected by the European Community Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Mollusks during 1995–2004 (17) and the second by the Food-Borne Viruses in Europe (FBVE) network, which has conducted surveillance for norovirus outbreaks in Europe since 1999. Prior investigation of the FBVE database of systematically collected epidemiologic and microbiological norovirus surveillance data (6) showed that the epidemiology of norovirus outbreaks in Europe varies between genogroups. An analysis of the properties of reported outbreaks indicated a clear difference between GII.4 strains and other noroviruses; non-GII.4 strains were found more frequently in outbreaks with a foodborne mode of transmission, and GII.4 strains were found more frequently in healthcare settings with person-to-person transmission (18,19). Here we demonstrate that further specification into genotypes shows additional differences in the epidemiology of norovirus outbreaks.
We used 2 broad databases reflecting norovirus prevalence within the European countries under surveillance. These databases provided us the opportunity to compare genotype proportions as detected in outbreaks, i.e., human surveillance data, with those detected in source-contaminated food products, i.e., bivalve mollusks monitoring data.
Human Surveillance Data
From January 1999 through December 2004, FBVE collected molecular information on 2,727 norovirus outbreaks and sporadic cases in Denmark, Finland, France, Germany, England and Wales, Hungary, Ireland, Italy, the Netherlands, Norway, Sweden, Slovenia, and Spain (20,21). Although the name FBVE suggested a foodborne focus, the network actually investigated outbreaks from all modes of transmission to obtain a comprehensive overview of viral activity in the community (strengths and limitations of the FBVE data collection were described by Kroneman et al. ; to compare newly detected strains with the FBVE database and find potential linked outbreaks, we used a comparison tool [www.rivm.nl/bnwww]). Data were reported to FBVE at outbreak level; therefore, no informed consent was needed. Outbreaks were categorized as follows on the basis of the cause of infection as reported in the surveillance system:
Foodborne-food (FB-food) when an outbreak was reported to be caused by food and the outbreak strain was detected in food;
Foodborne-feces (FB-feces) when an outbreak was reported to be caused by food and the outbreak strain was detected in human feces only;
Foodborne (FB) when an outbreak was classified as FB-food or FB-feces;
Food handler–borne (FHB) when an outbreak was reported to be caused by an infected food handler contaminating the food and the outbreak strain was detected in human feces;
Person-borne (PB) when an outbreak was reported to be caused by person-to-person transmission and the outbreak strain was detected in human feces;
Unknown (UN) when the mode of transmission was not reported or was reported to be unknown and the outbreak strain was detected in human feces.
When the mode of transmission was not reported but information was given in text data fields, this information was used to categorize the outbreak. Because we were interested in the origin of the virus, we categorized outbreaks involving PB transmission but starting with food as FB-food, FB-feces, or FHB, depending on available information. Strains detected in sporadic cases were clustered into outbreaks if information was available. The remaining strains detected in sporadic cases were considered of interest with respect to the genotypes causing human illness and representative of potential unreported outbreaks. When we detected multiple genotypes during an outbreak or in sporadic cases, we recorded each genotype.
Bivalve Mollusk Monitoring Data
The European Community Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Mollusks systematically collected sequence data on norovirus strains routinely detected in bivalve mollusks in Europe. During January 1999–December 2004, the laboratory systematically collected 295 strain sequences with region A sequence lengths varying from 76 to 78 nt. These strain sequences were detected as part of production area monitoring studies or outbreak investigations of gastroenteritis in Denmark, England and Wales, Ireland, Scotland, and Spain. All samples were first routinely tested with GI and GII PCR methods; then all positive samples were cloned (22), resulting in a representative reflection of norovirus presence in bivalve shellfish. If we detected multiple genotypes in 1 sample, we recorded each genotype.
Assignment of Genotypes
Strains were genotyped by using a previously described method for sequence analysis of a fragment of the RNA-dependent RNA polymerase gene regions B, C, and D (23) because these regions were used in the FBVE network. From the start, the network used sequence-based genotyping of the then most commonly used diagnostic PCR fragment, targeting the RNA-dependent RNA polymerase gene. Since then, however, it has become clear that recombination is common and mainly occurs in the area between the overlap between the polymerase and the capsid gene. Therefore, capsid-based and polymerase-based typing may be discordant. Genotype assignment was therefore performed only after clustering of query strains against all relevant available sequences in the FBVE database (M. Koopmans et al., unpub. data). This process resulted in the genotyping of all but 68 (2%) strains. Genotypes were classified on the basis of their similarity to reference strains representing known genotypes by using the norovirus typing library (www.noronet.nl/nov_quicktyping). If the (clustered) genotypes occurred <5 times in our 5-year covering data selection, the frequencies were considered too low to be ascribed a separate genotype and excluded. This was the situation for GII.18 and 6 clusters of nonassigned GII strains (n = 25, 1%).
First, we compared the genotype frequency distributions detected in outbreak categories reported as FB-food, FB-feces, FHB, PB, and UN and in routinely tested bivalve mollusks. To evaluate the correlation and measures of association of these 6 proportional profiles, Pearson correlation coefficient ρ was calculated on the basis of frequencies (ρ1) and logarithm (ρ2) of the frequencies of 22 genotypes, as well as Cramer V and simulated p values by using 20,000 replications with the exact variant of the χ2 test. The exploratory technique correspondence analysis allows for examining the structure of categorical variables in a multiway table and was used to visualize the measure of correspondence in the 6 genotype profiles. p values <0.05 were considered significant.
Second, to differentiate the remaining genotype profiles detected in outbreaks, we used the genotype profiles of the 2 main transmission modes to be distinguished during an outbreak investigation. For each genotype in the human surveillance collection, the fraction of outbreaks of known origin being FB (i.e., FB-food and FB-feces) or PB was estimated on the basis of the proportion of FB outbreaks of all FB + PB outbreaks in each genotype. We used the estimated proportion of FB outbreaks of all FB + PB outbreaks in each genotype to estimate the probability that an FHB or UN outbreak was foodborne. We calculated 95% confidence intervals (CIs) using Monte Carlo simulation with 10,000 random draws from the β distributions, which are the posterior probabilities of the proportions (24).
Of 3,022 detected noroviruses, 25 (1%) were excluded because of low frequencies; for 68 (2%), assignment of a genotype was not possible because of short sequences or inability of the method applied to type the detected norovirus beyond its genogroup. Of the remaining 2,929 strains, 71 (2%) could not be linked to epidemiologic data, and therefore their origin remained unknown, leaving 2,858 (95%) strains for analysis: 922 originating from PB outbreaks, 24 from FB-food outbreaks, 151 FB-feces outbreaks, 20 FHB outbreaks, 1,446 UN outbreaks, and all 295 bivalve mollusk monitoring strains. Among the outbreaks of known origin, 175 (16%) of 1,117 were reported to be FB (i.e., FB-food and FB-feces).
The proportion of genogroup I was significantly higher in bivalve mollusks (137/295, 46%) than in infected humans (313/2,539, 12%) (Table 1). All genotypes detected in bivalve mollusks were also detected in humans; however, 9 genotypes causing human illness were not detected in bivalve mollusks. Overall, the II.4 genotype was responsible for most of the human outbreaks (1,326/2,539, 52%), followed by II.b (328/2,539, 13%) and II.7 (156/2,539, 6%).
We visualized genotype frequency distributions as profiles for the observed categories of outbreaks and sorted them for their relevance in UN outbreaks, presented with different scales allowing for proportional comparison (Appendix Figure 1). The genotype profiles vary between these groups. The correlation coefficients based on frequencies, ρ1, showed that 2 genotype profiles were distinguishable (Table 2): 1 profile typically seen in human feces (FB-feces, FHB, or PB), and another profile typically detected in sources other than human feces, i.e., in food (FB-food) or bivalve mollusks. The ρ1 reflects some genotypes frequently and others rarely seen in FB-food and bivalve mollusks. Because FB-food strains include oyster-related outbreaks as well, we assumed that the correlation between FB-food and bivalve mollusks can be explained partly by these oyster-related outbreaks. We therefore calculated an additional correlation coefficient using the 14 strains detected in food items other than bivalve mollusks. Despite low numbers, this calculation resulted in a high, significant correlation coefficient (ρ = 0.81, p <0.001). The logarithm of the frequencies, ρ2 (Table 2), is less sensitive to peak frequencies of genotypes and therefore capable of differentiating profiles with respect to the rare genotypes and approaching the Cramer V. Cramer V and ρ2 show less clear association of profiles, with diverging results for the FHB and UN profiles.
Table 2 shows the quantification of association; the associated genotype profiles illustrated by correspondence analysis is shown in the Figure. The values of the 6 columns in Table 1 can be considered coordinates in a 6-dimensional space, and the distances are computed. These distances summarize information about the similarity between the rows in Table 1. Dimension 1 may be considered to differentiate transmission modes explaining 59.12% of the correspondence, confirming that the profiles found in bivalve mollusks and FB-food are similar with regard to the pattern of relative frequencies in genotypes (rows in Table 1) and differ from those in PB. It also shows that the FHB, UN, and FB-feces profiles are mutually similar, with their distance somewhere between the PB and FB-food/bivalve mollusk profiles. Dimension 2 may represent dual origin, explaining an additional 31.40%, showing that FB-feces, FHB, and UN mutually correspond and differ from FB-food, bivalve mollusks, and PB that mutually correspond.
When we compared the proportions of genotypes detected in FB outbreaks with those in PB outbreaks, we detected genotypes I.2 and I.4 significantly more frequently in FB outbreaks (Appendix Figure 2). On the other hand, genotypes I.6, II.1W, II.2, II.4, II.b, II.c, and II.d were detected significantly more frequently in PB outbreaks. Using these proportional FB and PB genotype profiles and their confidence intervals to distinguish between FB and PB transmission among 20 FHB outbreaks, we could ascribe 5 (95% CI 4–6) to FB and 15 (95% CI 14–16) to PB transmission. Ascribing 1,446 unexplained human norovirus outbreaks to either FB or PB transmission resulted in ≈367 (95% CI 327–417) FB outbreaks and ≈1,079 (95% CI 1,026–1,120) PB outbreaks. Overall, use of the genotype patterns increases the estimated number of FB proportion of outbreaks to 21% (547/2,563; range 20%–23%) compared with the 16% previously mentioned among the outbreaks of known origin.
Our combined epidemiologic and virologic analysis demonstrated that norovirus genotype profiles, derived from long-term norovirus strain collections, can be used to differentiate foodborne outbreaks caused by food contamination early in the food chain from those caused by food handlers contaminating food. Our study is one step in deriving practical applicable information from the existing record and possible only through the availability of continuously updated databases containing detailed epidemiologic data and virus characterization. We confirmed a significant difference in the GI:GII ratio; GI strains were more prevalent in bivalve mollusks. On the basis of the 5-year strain collections, some genotypes (I.2 and I.4) suggest FB instead of PB preference, and others (II.2 and II.6/II.7) are commonly seen in outbreaks but not detected in bivalve mollusks (and FB-food). Strains detected in food that caused outbreaks (FB-food) showed a genotype profile similar to those in bivalve mollusk monitoring and dissimilar to the profile detected in human feces (i.e., FB-feces, FHB, PB, UN) with respect to the frequently seen genotypes. This finding may reflect the ability of these genotypes to survive outside humans or their diminished ability to spread or replicate within the human population. Genotype profiles of FHB and UN resulted in diverging association outcomes, which may reflect their potential dual origin.
Although consumption of contaminated food causes both types of outbreaks, outbreaks resulting from infected food handlers clearly necessitate different measures than do outbreaks resulting from food contaminated early in the food chain. Consequently, differentiation of these modes of transmission is of interest to food safety authorities and public health institutions. Food handler–borne outbreaks are end-of-chain outbreaks easily recognized as such, as numerous outbreak reports illustrate (25–29). Such outbreaks can be prevented or limited by exclusion of infected or shedding food handlers from work until 48–72 hours after recovery (25,27,29,30), education of food handlers (26), and standard testing of food handlers during outbreaks (28). A common source of contamination early in the food chain, however, may be more difficult to detect. Such contamination may result from sewage influx containing multiple viruses (8,9,31), making a link difficult to identify (31). Moreover, sewage most likely contains noroviruses from person-to-person outbreaks, which can contaminate the food and thereby dilute the genotype profiling effect. Use of genotype profiles is a first step toward recognizing outbreaks resulting from contamination early in the food chain because it allows estimation of the incidence in surveillance data retrospectively and objectively minimizes misclassification of outbreaks. However, genotyping data need to be interpreted with care, and continuous updating of the database remains necessary.
Our study has some limitations. First, our measures of association could not detect differences between genotype profiles with respect to the rare genotypes. Even so, the rare outbreak or sporadic strains are of interest because they may represent potential emerging or zoonotic genotypes with consequences for public health. Types that were initially rare may remain in human surveillance, as seen with the emergence of GII.b after a large waterborne outbreak (10) followed by, among others, foodborne distribution throughout Europe. Since then, GII.b strains have caused 13% of all outbreaks (Table 1), now mainly PB, suggesting good adaptation. On the other hand, if the rare types are unable to adapt for persistence in the human population, they may be repeatedly reintroduced, causing only sporadic cases but not outbreaks. This repeated introduction of sporadic cases would remain undetected at present because routine surveillance for sporadic cases is rare (32) and is not the current practice of FBVE. To identify the origin of newly emerging and rare strains, systematic monitoring of additional potential sources, such as cattle and swine (33) as well as sporadic human cases, is necessary.
Second, in our analysis, the transmission route was reported as unknown for 57% of outbreak strains. Incompleteness of surveillance data is a common problem (34) and has been recognized in surveillance of foodborne viral infections (35), including in the FBVE database (19,20). Incomplete data may have resulted in underestimation of the number of foodborne outbreaks because they may be complicated to identify. Food safety authorities routinely confirm FB clusters by detecting pathogens in food, but such confirmation is difficult for viruses because viruses, unlike bacteria, do not replicate in food, resulting in a low viral load for extraction and concentration. In addition, the matrix involved may complicate these procedures, and successful detection methods are available primarily for fresh produce with surface contamination and virus-accumulating shellfish (36,37). However, knowledge of the prevalence of strains in the environment, foods, and humans is necessary for the interpretation of matching. Such knowledge requires monitoring, which is limited to shellfish and norovirus outbreaks (38). For monitoring of foods other than shellfish, methods sensitive enough to detect viruses in naturally contaminated (and not spiked) food are required. The technical advisory group (TAG 4) of the Viruses in Food workgroup (WG 6) in the Technical Committee of Horizontal Methods for Food Analysis (TC 275) of the European Committee for Standardization (CEN) is validating standard methods for norovirus detection in bivalve mollusks, soft fruit, leafy vegetables, and bottled water (39). Until such methods are available and provide knowledge about the prevalence of viral presence in foods, the use of genetic profiles retrospectively derived from outbreak surveillance data is likely to improve foodborne viral surveillance. Because the norovirus strain population is continuously evolving, our analysis needs to be repeated periodically to ensure that retrospective findings remain predictive.
Third, international comparison of norovirus strains is complicated because of their genetic diversity and the involvement of several laboratories in diagnosis; consequential different assays result in sequences with diverging lengths and from diverging genomic regions. However, this limitation is not likely to have influenced our results because it affects mostly the comparison of sequence clusters and not genotypes. Moreover, within FBVE, standardization of diagnostic methods occurs by having participating laboratories regularly test a representative panel of fecal samples (40).
We showed that norovirus genotype profiles can be used to estimate the foodborne proportion of norovirus outbreaks while excluding those of the food handler as a source. Distinction at genogroup level had already indicated epidemiologic differences (19), and we have now demonstrated that genotype profiles can be used to differentiate transmission modes. The profiles and proportions are likely to be helpful for estimating the number of outbreaks with potential of causing geographically disseminated outbreaks. Because identification and investigation of such outbreaks provides insight into effective prevention measures during the production process, detection should enable containment of viral foodborne infection and thus prevent further spread and the consequent potential for large numbers of human infections.
Ms Verhoef is an epidemiologist in the Virology Division of the Diagnostic Laboratory for Infectious Diseases in the National Institute for Public Health and the Environment. Her work focuses on the epidemiology and surveillance of infectious diseases, particularly noroviruses.
We thank Annelies Kroneman, Soizick Le Guyader, and Leena Maunula for critically reviewing the manuscript and Amal Chatterjee for editorial assistance.
This work was supported by the Dutch Food and Consumer Product Safety Authority, the European Commission Directorate General Research Quality of Life Program, 6th Framework (EVENT, SP22-CT-2004-502571) and Secretariat General SANCO (DIVINE-net, 2003213).
- Cannon JL, Papafragkou E, Park GW, Osborne J, Jaykus LA, Vinje J. Surrogates for the study of norovirus stability and inactivation in the environment: a comparison of murine norovirus and feline calicivirus. J Food Prot. 2006;69:2761–5.
- Dolin R, Blacklow NR, DuPont H, Formal S, Buscho RF, Kasel JA, Transmission of acute infectious nonbacterial gastroenteritis to volunteers by oral administration of stool filtrates. J Infect Dis. 1971;123:307–12.
- Duizer E, Koopmans M. Tracking emerging pathogens: the case of noroviruses. In: Motarjemi Y, Adams M, editors. Emerging food-borne pathogens. Boca Raton (FL): Woodhead Publishing; 2006. p. 77–110.
- Food and Agriculture Organization of the United Nations/World Health Organization. Viruses in food: scientific advice to support risk management activities. Microbiological Risk Assessment series no. 13. Rome: The Organization; 2008.
- Greening G. Human and animal viruses in food (including taxonomy of enteric viruses). In: Goyal SM, editor. Viruses in foods. New York (NY): Springer; 2006. p. 5–42.
- Koopmans M, Vennema H, Heersma H, van Strien E, van Duynhoven Y, Brown D, Early identification of common-source foodborne virus outbreaks in Europe. Emerg Infect Dis. 2003;9:1136–42.
- Falkenhorst G, Krusell L, Lisby M, Madsen SB, Bottiger B, Molbak K. Imported frozen raspberries cause a series of norovirus outbreaks in Denmark, 2005. Euro Surveill. 2005;10:pii2795 [cited 2005 Sep 22]. http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=2795
- Le Guyader FS, Bon F, DeMedici D, Parnaudeau S, Bertone A, Crudeli S, Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption. J Clin Microbiol. 2006;44:3878–82.
- Webby RJ, Carville KS, Kirk MD, Greening G, Ratcliff RM, Crerar SK, Internationally distributed frozen oyster meat causing multiple outbreaks of norovirus infection in Australia. Clin Infect Dis. 2007;44:1026–31.
- Gallay A, De Valk H, Cournot M, Ladeuil B, Hemery C, Castor C, A large multi-pathogen waterborne community outbreak linked to faecal contamination of a groundwater system, France, 2000. Clin Microbiol Infect. 2006;12:561–70.
- Buesa J, Collado B, Lopez-Andujar P, Abu-Mallouh R, Rodriguez Diaz J, Garcia Diaz A, Molecular epidemiology of caliciviruses causing outbreaks and sporadic cases of acute gastroenteritis in Spain. J Clin Microbiol. 2002;40:2854–9.
- Koopmans M, Lopman B, Vennema H, Reacher M, Carrique-Mas J, Van Duijnhoven Y, Foodborne viruses in Europe: web-based technologies for investigation of transnational outbreaks of viral gastroenteritis. Proceedings of the International Conference on Emerging Infectious Diseases. 2002 Mar 24–27. Atlanta, GA, USA.
- Hald T, Vose D, Wegener HC, Koupeev T. A Bayesian approach to quantify the contribution of animal-food sources to human salmonellosis. Risk Anal. 2004;24:255–69.
- Boxman IL, Tilburg JJ, te Loeke NA, Vennema H, de Boer E, Koopmans M. An efficient and rapid method for recovery of norovirus from food associated with outbreaks of gastroenteritis. J Food Prot. 2007;70:504–8.
- Le Guyader FS, Mittelholzer C, Haugarreau L, Hedlund KO, Alsterlund R, Pommepuy M, Detection of noroviruses in raspberries associated with a gastroenteritis outbreak. Int J Food Microbiol. 2004;97:179–86.
- Rutjes SA, Lodder Verschoor F, van der Poel WH, van Duijnhoven YT, de Roda Husman AM. Detection of noroviruses in foods: a study on virus extraction procedures in foods implicated in outbreaks of human gastroenteritis. J Food Prot. 2006;69:1949–56.
- Green J, Henshilwood K, Gallimore CI, Brown DW, Lees DN. A nested reverse transcriptase PCR assay for detection of small round-structured viruses in environmentally contaminated molluscan shellfish. Appl Environ Microbiol. 1998;64:858–63.
- Kroneman A, Verhoef L, Harris J, Vennema H, Duizer E, van Duynhoven Y, Analysis of integrated virological and epidemiological reports of norovirus outbreaks collected within the foodborne viruses in Europe Network from 1 July 2001 to 30 June 2006. J Clin Microbiol. 2008;46:2959–65.
- Verhoef LP, Kroneman A, Van Duijnhoven Y, Boshuizen H, van Pelt W, Koopmans M. Selection tool for foodborne norovirus outbreaks. Emerg Infect Dis. 2009;15:31–8.
- Kroneman A, Harris J, Vennema H, Duizer E, van Duynhoven Y, Gray J, Data quality of 5 years of central norovirus outbreak reporting in the European Network for food-borne viruses. J Public Health (Oxf). 2008;30:82–90.
- Lopman BA, Reacher MH, Van Duijnhoven Y, Hanon FX, Brown D, Koopmans M. Viral gastroenteritis outbreaks in Europe, 1995–2000. Emerg Infect Dis. 2003;9:90–6.
- Henshilwood K, Green J, Lees DN. Monitoring the marine environment for small round structured viruses (SRSVs): a new approach to combating the transmission of these viruses by mulluscan shellfish. Water Sci Technol. 1998;38:51–6.
- Vinje J, Koopmans MP. Molecular detection and epidemiology of small round-structured viruses in outbreaks of gastroenteritis in the Netherlands. J Infect Dis. 1996;174:610–5.
- Brown LD, Cai TT, DasGupta A. Interval estimation for a binomial proportion. Stat Sci. 2001;16:101–33.
- Centers for Disease Control and Prevention. Multisite outbreak of norovirus associated with a franchise restaurant—Kent County, Michigan, May 2005. MMWR Morb Mortal Wkly Rep. 2006;55:395–7.
- Centers for Disease Control and Prevention. Norovirus outbreak associated with ill food-service workers—Michigan, January–February 2006. MMWR Morb Mortal Wkly Rep. 2007;56:1212–6.
- Friedman DS, Heisey Grove D, Argyros F, Berl E, Nsubuga J, Stiles T, An outbreak of norovirus gastroenteritis associated with wedding cakes. Epidemiol Infect. 2005;133:1057–63.
- Godoy P, Izcara J, Bartolome R, Bach P, Escobar A, Pal M, Outbreak of food-borne norovirus associated with the consumption of sandwiches [in Spanish]. Med Clin (Barc). 2005;124:161–4.
- Schmid D, Stuger HP, Lederer I, Pichler AM, Kainz Arnfelser G, Schreier E, A foodborne norovirus outbreak due to manually prepared salad, Austria 2006. Infection. 2007;35:232–9.
- Rooney RM, Cramer EH, Mantha S, Nichols G, Bartram JK, Farber JM, A review of outbreaks of foodborne disease associated with passenger ships: evidence for risk management. Public Health Rep. 2004;119:427–34.
- Gallimore CI, Pipkin C, Shrimpton H, Green AD, Pickford Y, McCartney C, Detection of multiple enteric virus strains within a foodborne outbreak of gastroenteritis: an indication of the source of contamination. Epidemiol Infect. 2005;133:41–7.
- Zintz C, Bok K, Parada E, Barnes-Eley M, Berke T, Staat MA, Prevalence and genetic characterization of caliciviruses among children hospitalized for acute gastroenteritis in the United States. Infect Genet Evol. 2005;5:281–90.
- Mattison K, Shukla A, Cook A, Pollari F, Friendship R, Kelton D, Human noroviruses in swine and cattle. Emerg Infect Dis. 2007;13:1184–8.
- Doyle TJ, Glynn MK, Groseclose SL. Completeness of notifiable infectious disease reporting in the United States: an analytical literature review. Am J Epidemiol. 2002;155:866–74.
- O’Brien S. The challenge of estimating the burden of an underreported disease. In: Koopmans M, Cliver DO, Bosch A, editors. Food-borne viruses. Washington (DC): ASM Press; 2008. p. 87–116.
- Atmar RL. Molecular methods of virus detection in food. In: Goyal SM, editor. Viruses in foods. New York: Springer 2006. p. 121–43.
- Goyal SM. Conventional methods of virus detection in foods. In: Goyal SM, editor. Viruses in foods. New York: Springer; 2006. p. 101–20.
- Verhoef L, Boxman I, Koopmans M. Viruses transmitted through the food-chain: a review of the latest developments. CAB Reviews. 2008;3:1–15.
- Costafreda MI, Bosch A, Pinto RM. Development, evaluation, and standardization of a real-time TaqMan reverse transcription–PCR assay for quantification of hepatitis A virus in clinical and shellfish samples. Appl Environ Microbiol. 2006;72:3846–55.
- Vinje J, Vennema H, Maunula L, von Bonsdorff CH, Hoehne M, Schreier E, International collaborative study to compare reverse transcriptase PCR assays for detection and genotyping of noroviruses. J Clin Microbiol. 2003;41:1423–33.
Suggested citation for this article: Verhoef L, Vennema H, van Pelt W, Lees D, Boshuizen H, Henshilwood K, et al. Use of norovirus genotype profiles to differentiate origins of foodborne outbreaks. Emerg Infect Dis [serial on the Internet]. 2010 Apr [date cited]. http://wwwnc.cdc.gov/eid/article/16/4/09-0723.htm
1Members of the Food-Borne Viruses in Europe Network: B. Böttiger, K. Mølbak, C. Johnsen (Denmark); K.-H. von Bonsdorff, L. Maunula, M. Kuusi (Finland); P. Pothier, K. Balay, J. Kaplon, G. Belliot, S. Le Guyader (France); E. Schreier, K. Stark, J. Koch, M. Höhne (Germany); G. Szücs, G. Reuter, K. Krisztalovics (Hungary); Ireland: M. Lynch, B. Foley, P. McKeown, S. Coughlan (Ireland); E. Duizer, A. Kroneman, Y. van Duynhoven (the Netherlands); K. Vainio, K. Nygard, G. Kapperud (Norway); M. Poljsak-Prijatelj, D. Barlic-Maganja, A. Hocevar Grom (Slovenia); F. Ruggeri, I. Di Bartolo (Italy); A Bosch, A. Dominguez, J. Buesa, A. Sanchez Fauquier, G. Hernández-Pezzi (Spain); K.-O. Hedlund, Y. Andersson, M. Thorhagen, M. Lysén, M. Hjertqvist (Sweden); D. Brown, B. Adak, J. Gray, J. Harris, M. Iturriza (United Kingdom).