La Crosse Virus in Aedes albopictus Mosquitoes, Texas, USA, 2009
Amy J. Lambert , Carol D. Blair, Mary D’Anton, Winnann Ewing, Michelle Harborth, Robyn Seiferth, Jeannie Xiang, and Robert S. Lanciotti
Author affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (A.J. Lambert, R.S. Lanciotti); Colorado State University, Fort Collins (C.D. Blair); Texas Department of State Health Services, Austin, Texas, USA (M. D’Anton, W. Ewing, M. Harborth, R. Seiferth, J. Xiang)
Figure 2. Phylogeny of La Crosse virus (LACV) medium (M) segment sequences of diverse origins. According to a limited availability of full-length sequences in GenBank, 1,663 nt of the M segment glycoprotein gene open-reading frame are compared. Isolate source and GenBank accession nos. appear after the isolate designation for each taxon. Sequences were aligned by ClustalW (10) and neighbor-joining and maximum-parsimony trees were generated by using 2,000 bootstrap replicates with MEGA version 4 software (10). Highly similar topologies and confidence values were derived by all methods and a neighbor-joining tree is shown. Scale bar represents the number of nucleotide substitutions per site. The 2009 Texas (TX) isolates group with strong support with lineage 2 viruses of the extreme south and New York (NY), which suggests a likely southern origin for LACV isolates. MN, Minnesota; WI, Wisconsin; Oc., Ochlerotatus; MO, Missouri; TN, Tennessee; Ae., Aedes; NC, North Carolina; OH, Ohio; WV, West Virginia; AL, Alabama; Ps., psorophora; GA, Georgia; CT, Connecticut.
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