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Volume 16, Number 5—May 2010

Dispatch

La Crosse Virus in Aedes albopictus Mosquitoes, Texas, USA, 2009

Amy J. LambertComments to Author , Carol D. Blair, Mary D’Anton, Winnann Ewing, Michelle Harborth, Robyn Seiferth, Jeannie Xiang, and Robert S. Lanciotti
Author affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (A.J. Lambert, R.S. Lanciotti); Colorado State University, Fort Collins (C.D. Blair); Texas Department of State Health Services, Austin, Texas, USA (M. D’Anton, W. Ewing, M. Harborth, R. Seiferth, J. Xiang)

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Figure 2

Phylogeny of La Crosse virus (LACV) medium (M) segment sequences of diverse origins. According to a limited availability of full-length sequences in GenBank, 1,663 nt of the M segment glycoprotein gene open-reading frame are compared. Isolate source and GenBank accession nos. appear after the isolate designation for each taxon. Sequences were aligned by ClustalW (10) and neighbor-joining and maximum-parsimony trees were generated by using 2,000 bootstrap replicates with MEGA version 4 software (

Figure 2. Phylogeny of La Crosse virus (LACV) medium (M) segment sequences of diverse origins. According to a limited availability of full-length sequences in GenBank, 1,663 nt of the M segment glycoprotein gene open-reading frame are compared. Isolate source and GenBank accession nos. appear after the isolate designation for each taxon. Sequences were aligned by ClustalW (10) and neighbor-joining and maximum-parsimony trees were generated by using 2,000 bootstrap replicates with MEGA version 4 software (10). Highly similar topologies and confidence values were derived by all methods and a neighbor-joining tree is shown. Scale bar represents the number of nucleotide substitutions per site. The 2009 Texas (TX) isolates group with strong support with lineage 2 viruses of the extreme south and New York (NY), which suggests a likely southern origin for LACV isolates. MN, Minnesota; WI, Wisconsin; Oc., Ochlerotatus; MO, Missouri; TN, Tennessee; Ae., Aedes; NC, North Carolina; OH, Ohio; WV, West Virginia; AL, Alabama; Ps., psorophora; GA, Georgia; CT, Connecticut.

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