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Volume 16, Number 5—May 2010


La Crosse Virus in Aedes albopictus Mosquitoes, Texas, USA, 2009

Amy J. LambertComments to Author , Carol D. Blair, Mary D’Anton, Winnann Ewing, Michelle Harborth, Robyn Seiferth, Jeannie Xiang, and Robert S. Lanciotti
Author affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (A.J. Lambert, R.S. Lanciotti); Colorado State University, Fort Collins (C.D. Blair); Texas Department of State Health Services, Austin, Texas, USA (M. D’Anton, W. Ewing, M. Harborth, R. Seiferth, J. Xiang)

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Orthobunyavirus consensus oligonucleotide primers used for amplification and sequencing of La Crosse virus partial S, M, and L segment cDNAs, Texas, 2009*

Targeted genomic regions Name Primer sequence (5′ → 3′) Approximate amplicon size, bp
S segment nucleocapsid ORF Cal S forward GCAAATGGATTTGATCCTGATGCAG 210

Cal S reverse

M segment 5′ terminus/glycoprotein ORF Ortho M 5′ terminus AGTAGTGTACTACC 410

Ortho M ORF reverse

L segment 5′ terminus/polymerase ORF Ortho L 5′ terminus AGTAGTGTACTCCTA 550

*Oligonucleotide primers designed against conserved regions of the orthobunyavirus genome. S segment primers appear in a previous publication (9). All primers were applied in singleplex reactions using methods described previously (9) with altered primer annealing conditions of 50oC for 1 min. S, small; M, medium; L, large; ORF, open reading frame.

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