Volume 18, Number 1—January 2012
Research
Assessing Prion Infectivity of Human Urine in Sporadic Creutzfeldt-Jakob Disease
Silvio Notari1, Liuting Qing1, Maurizio Pocchiari, Ayuna Dagdanova, Kristin Hatcher, Arend Dogterom, Jose F. Groisman, Ib Bo Lumholtz, Maria Puopolo, Corinne Lasmezas, Shu G. Chen, Qingzhong Kong, and Pierluigi Gambetti
Table 1
Infectivity titers in microsomal fraction of 4 patients with sCJDMM1*
| sCJDMM1 inoculum | Brain† dilution | Incubation time, d , mean ± SEM | Distribution | Prion titer, ID50/g tissue† |
|---|---|---|---|---|
| Patient 1 | 10−1 | 256 ± 5 | 7/7 | 3.0 × 106 |
| 10−2 | 297 ± 18 | 4/4 | ||
| 10−3 | 314 ± 15 | 5/5 | ||
| 10−4 | 339 ± 16 | 5/5 | ||
| 10−5 | 420, 437 | 2/5 | ||
| 10−6 | >681 | 0/5 | ||
| Patient 2 | 10−4 | 382 ± 12 | 5/10 | 2.2 × 106 |
| Patient 3 | 10−4 | 348 ± 12 | 10/10 | 1.2 × 107 |
| Patient 4 | 10−2 | 267 ± 14 | 5/5 | 7.2 × 106 |
*sCJD, sporadic Creutzfeldt-Jakob disease; ID50, 50% infectious dose.
†Brain tissue equivalent.
1These authors contributed equally to this article.
