Volume 18, Number 1—January 2012
Research
Assessing Prion Infectivity of Human Urine in Sporadic Creutzfeldt-Jakob Disease
Silvio Notari1, Liuting Qing1, Maurizio Pocchiari, Ayuna Dagdanova, Kristin Hatcher, Arend Dogterom, Jose F. Groisman, Ib Bo Lumholtz, Maria Puopolo, Corinne Lasmezas, Shu G. Chen, Qingzhong Kong, and Pierluigi Gambetti
Table 2
Prion infectivity in microsomal fraction prepared from sCJDMM1 patient 4 and suspended in PBS or 100× concentrated and dialyzed urine*
| Carrier | Brain† dilution | Incubation time, d, mean ± SEM | Distribution | Prion titer, ID50/g tissue† |
|---|---|---|---|---|
| PBS‡ | 10−2 | 267 ± 14 | 5/5 | 7.2 × 106 |
| 100× concentrated and dialyzed normal human urine | 10−1 | 262 ± 4 | 5/5 | 3.3 × 105 |
| 10−2 | 281 ± 7 | 5/5 | ||
| 10−3 | 377 ± 35 | 6/6 | ||
| 10−4 | 311, 335 | 2/4 | ||
| 10−5 | >793 | 0/6 | ||
| 10−6 | >702 | 0/6 |
*sCJD, sporadic Creutzfeldt-Jakob disease; PBS, phosphate-buffered saline; ID50, 50% infectious dose.
†Brain tissue equivalent.
‡Titer was the same as calculated in Table 1.
1These authors contributed equally to this article.
