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Volume 18, Number 10—October 2012

Research

WU and KI Polyomaviruses in Respiratory Samples from Allogeneic Hematopoietic Cell Transplant Recipients

Jane Kuypers1Comments to Author , Angela P. Campbell1, Katherine A. Guthrie, Nancy L. Wright, Janet A. Englund, Lawrence Corey, and Michael Boeckh
Author affiliations: University of Washington, Seattle, Washington, USA (J. Kuypers, A.P. Campbell, N.L. Wright, J.A. Englund, L. Corey, M. Boeckh); Fred Hutchinson Cancer Research Center, Seattle (J. Kuypers, A.P. Campbell, K.A. Guthrie, J.A. Englund, L. Corey, M. Boeckh); and Seattle Children’s Hospital, Seattle (A.P. Campbell, J.A. Englund)

Main Article

Table 1

Primers and probes used for detecting KIPyV, WUPyV, and 2 other viruses by real-time RT-PCR and PCR in HCT recipients*

Virus Target gene Amplicon size, bp Function Sequence/label, 5′→3′ PCR concentration, nmol/L
KIPyV VP2–3 74 Forward primer CTATCCCTGAATACCAGTTGGAAAC 425
Reverse primer GTATGACGCGACAAGGTTGAAG 425
Probe FAM-TTCCGGGCATCCCAGACTGGC-BHQ1 125
WUPyV VP1 75 Forward primer AACCAGGAAGGTCACCAAGAAG 300
Reverse primer TCTACCCCTCCTTTTCTGACTTGT 300
Probe HEX-CAACCCACAAGAGTGCAAAGCCTTCC-BHQ1 75
Influenza B Matrix 76 Forward primer CACAATTGCCTACCTGCTTTCA 250
Reverse primer CCAACAGTGTAATTTTTCTGCTAGTTCT 250
Probe VIC-CTTTGCCTTCTCCATCTT-MGBNFQ 100
Parainfluenza type 4 NP 94 Forward primer TGCCAAATCGGCAATAAACA 250
Reverse primer GGCTCTGGCAGCAATCATAAG 250
Probe VIC-TGATTCTGCATTGATGTGG-MGBNFQ 100

*KIPyV, KI polyomavirus; WUPyV, WU polyomavirus; RT-PCR, reverse transcription PCR; HCT, hematopoietic cell transplantation; VP, viral protein; NP, nucleoprotein.

Main Article

1These authors contributed equally to this article.

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